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AIP Recombinant Rabbit Monoclonal Antibody [PSH03-17] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750858

AIP Recombinant Rabbit Monoclonal Antibody [PSH03-17] - BSA and Azide free

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

AIP Recombinant Rabbit Monoclonal Antibody [PSH03-17] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human AIP aa 1-330 / 330.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 38 kDa

Positive Control

HeLa cell lysate, Jurkat cell lysate, HEK-293 cell lysate, THP-1 cell lysate, HCT 1 cell lysate, MCF7 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate.

Conjugation

unconjugated

Clone Number

PSH03-17

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

Target

Function

The protein encoded by this gene is a receptor for aryl hydrocarbons and a ligand-activated transcription factor. The encoded protein is found in the cytoplasm as part of a multiprotein complex, but upon binding of ligand is transported to the nucleus. This protein can regulate the expression of many xenobiotic metabolizing enzymes. Also, the encoded protein can bind specifically to and inhibit the activity of hepatitis B virus. Three transcript variants encoding different isoforms have been found for this gene. May play a positive role in AHR-mediated (aromatic hydrocarbon receptor) signaling, possibly by influencing its receptivity for ligand and/or its nuclear targeting.

Background References

1. Cai F, Hong Y, Xu J, Wu Q, Reis C, Yan W, Wang W, Zhang J. A Novel Mutation of Aryl Hydrocarbon Receptor Interacting Protein Gene Associated with Familial Isolated Pituitary Adenoma Mediates Tumor Invasion and Growth Hormone Hypersecretion. World Neurosurg. 2019 Mar;123:e45-e59.

2. Kuzhandaivelu N, Cong YS, Inouye C, Yang WM, Seto E. XAP2, a novel hepatitis B virus X-associated protein that inhibits X transactivation. Nucleic Acids Res. 1996 Dec 1;24(23):4741-50.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: O00170 Human

SwissProt: O08915 Mouse

SwissProt: Q5FWY5 Rat

Synonyms

AH receptor interacting protein antibody

AH receptor-interacting protein antibody

AIP antibody

AIP_HUMAN antibody

ARA 9 antibody

ARA9 antibody

Aryl hydrocarbon receptor interacting protein antibody

Aryl-hydrocarbon receptor-interacting protein antibody

fa03h10 antibody

FKBP 16 antibody

Expand

Images

  • Western blot analysis of AIP on different lysates with Rabbit anti-AIP antibody (<a href="/products/HA750858" style="font-weight: bold;text-decoration: underline;">HA750858</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2:Jurkat cell lysate (20 µg/Lane)<br />Lane 3: HEK-293 cell lysate (20 µg/Lane)<br />Lane 4: THP-1 cell lysate (20 µg/Lane)<br />Lane 5: HCT 116 cell lysate (20 µg/Lane)<br />Lane 6: MCF7 cell lysate (20 µg/Lane)<br />Lane 7: RAW264.7 cell lysate (20 µg/Lane)<br />Lane 8: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 9: C6 cell lysate (20 µg/Lane)<br />Lane 10: Mouse spleen tissue lysate (40 µg/Lane)<br />Lane 11: Rat spleen tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 38 kDa<br />Observed band size: 35 kDa<br /><br />Exposure time: 1 minutes 2 seconds;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750858" style="font-weight: bold;text-decoration: underline;">HA750858</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of AIP on different lysates with Rabbit anti-AIP antibody (HA750858) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2:Jurkat cell lysate (20 µg/Lane)
    Lane 3: HEK-293 cell lysate (20 µg/Lane)
    Lane 4: THP-1 cell lysate (20 µg/Lane)
    Lane 5: HCT 116 cell lysate (20 µg/Lane)
    Lane 6: MCF7 cell lysate (20 µg/Lane)
    Lane 7: RAW264.7 cell lysate (20 µg/Lane)
    Lane 8: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 9: C6 cell lysate (20 µg/Lane)
    Lane 10: Mouse spleen tissue lysate (40 µg/Lane)
    Lane 11: Rat spleen tissue lysate (40 µg/Lane)

    Predicted band size: 38 kDa
    Observed band size: 35 kDa

    Exposure time: 1 minutes 2 seconds;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750858) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of AIP on different lysates with Rabbit anti-AIP antibody (<a href="/products/HA750858" style="font-weight: bold;text-decoration: underline;">HA750858</a>) at 1/5,000 dilution.<br /><br />Lane 1: A549-WT cell lysate (10 µg/Lane)<br />Lane 2: A549-KD AIP cell lysate (10 µg/Lane)<br /><br />Predicted band size: 38 kDa<br />Observed band size: 35 kDa<br /><br />Exposure time: 2 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750858" style="font-weight: bold;text-decoration: underline;">HA750858</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of AIP on different lysates with Rabbit anti-AIP antibody (HA750858) at 1/5,000 dilution.

    Lane 1: A549-WT cell lysate (10 µg/Lane)
    Lane 2: A549-KD AIP cell lysate (10 µg/Lane)

    Predicted band size: 38 kDa
    Observed band size: 35 kDa

    Exposure time: 2 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750858) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"