Ninein Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH03-23] (HA750863)
Catalog# HA750863
Rabbit Monoclonal to Ninein
Tested for
-
WB
Reacts with
-
Human
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Ninein on different lysates with Rabbit anti-Ninein antibody (HA750863) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: A549 cell lysate
Lane 3: HT-29 cell lysate
Lane 4: MEF cell lysate
Lane 5: Human lung tissue lysate
Lane 6: Mouse lung tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 243 kDa
Observed band size: 270 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750863) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: A549 cell lysate
Lane 3: HT-29 cell lysate
Lane 4: MEF cell lysate
Lane 5: Human lung tissue lysate
Lane 6: Mouse lung tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 243 kDa
Observed band size: 270 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750863) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.