Ninein Recombinant Rabbit Monoclonal Antibody [PSH03-23] - BSA and Azide free
Catalog# HA750863
Ninein Recombinant Rabbit Monoclonal Antibody [PSH03-23] - BSA and Azide free
-
WB
-
Human
-
Mouse
-
unconjugated
Overview
Product Name
Ninein Recombinant Rabbit Monoclonal Antibody [PSH03-23] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Ninein aa 1-500 / 2,090.
Species Reactivity
Human, Mouse
Validated Applications
WB
Target Molecular Weight
Predicted band size: 243 kDa
Positive Control
HeLa cell lysate, A549 cell lysate, HT-29 cell lysate, MEF cell lysate, human lung tissue lysate, mouse lung tissue lysate.
Conjugation
unconjugated
Clone Number
PSH03-23
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Ninein, together with its paralog Ninein-like protein is one of the proteins important for centrosomal function. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells.
Background References
1. He L et al. PTRN-1 (CAMSAP) and NOCA-2 (NINEIN) are required for microtubule polarity in Caenorhabditis elegans dendrites. PLoS Biol. 2022 Nov
2. Cao Z et al. β3-Endonexin interacts with ninein in vascular endothelial cells to promote angiogenesis. Biochem Biophys Res Commun. 2021 Aug
Subcellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole.
Synonyms
Centrosome Marker antibody
Glycogen synthase kinase 3 beta interacting protein antibody
GSK3B interacting protein antibody
h Glycogen synthase kinase 3 beta-interacting protein antibody
hNinein antibody
KIAA1565 antibody
NIN antibody
NIN protein antibody
NIN_HUMAN antibody
ninein (GSK3B interacting protein) antibody
ExpandImages
-
This data was developed using HA721965, the same antibody clone in a different buffer formulation.
Western blot analysis of Ninein on different lysates with Rabbit anti-Ninein antibody (HA721965) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: A549 cell lysate
Lane 3: HT-29 cell lysate
Lane 4: MEF cell lysate
Lane 5: Human lung tissue lysate
Lane 6: Mouse lung tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 243 kDa
Observed band size: 270 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721965) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"