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Ninein Recombinant Rabbit Monoclonal Antibody [PSH03-23] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750863

Ninein Recombinant Rabbit Monoclonal Antibody [PSH03-23] - BSA and Azide free

Application

  • WB

Reactivity

  • Human

  • Mouse

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Ninein Recombinant Rabbit Monoclonal Antibody [PSH03-23] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Ninein aa 1-500 / 2,090.

Species Reactivity

Human, Mouse

Validated Applications

WB

Molecular Weight

Predicted band size: 243 kDa

Positive Control

HeLa cell lysate, A549 cell lysate, HT-29 cell lysate, MEF cell lysate, human lung tissue lysate, mouse lung tissue lysate.

Conjugation

unconjugated

Clone Number

PSH03-23

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

Target

Function

Ninein, together with its paralog Ninein-like protein is one of the proteins important for centrosomal function. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells.

Background References

1. He L et al. PTRN-1 (CAMSAP) and NOCA-2 (NINEIN) are required for microtubule polarity in Caenorhabditis elegans dendrites. PLoS Biol. 2022 Nov

2. Cao Z et al. β3-Endonexin interacts with ninein in vascular endothelial cells to promote angiogenesis. Biochem Biophys Res Commun. 2021 Aug

Subcellular Location

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole.

UNIPROT

SwissProt: Q8N4C6 Human

SwissProt: Q61043 Mouse

Synonyms

Centrosome Marker antibody

Glycogen synthase kinase 3 beta interacting protein antibody

GSK3B interacting protein antibody

h Glycogen synthase kinase 3 beta-interacting protein antibody

hNinein antibody

KIAA1565 antibody

NIN antibody

NIN protein antibody

NIN_HUMAN antibody

ninein (GSK3B interacting protein) antibody

Expand

Images

  • Western blot analysis of Ninein on different lysates with Rabbit anti-Ninein antibody (<a href="/products/HA750863" style="font-weight: bold;text-decoration: underline;">HA750863</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: A549 cell lysate<br />Lane 3: HT-29 cell lysate<br />Lane 4: MEF cell lysate<br />Lane 5: Human lung tissue lysate<br />Lane 6: Mouse lung tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 243 kDa<br />Observed band size: 270 kDa<br /><br />Exposure time: 5 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750863" style="font-weight: bold;text-decoration: underline;">HA750863</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Ninein on different lysates with Rabbit anti-Ninein antibody (HA750863) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: A549 cell lysate
    Lane 3: HT-29 cell lysate
    Lane 4: MEF cell lysate
    Lane 5: Human lung tissue lysate
    Lane 6: Mouse lung tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 243 kDa
    Observed band size: 270 kDa

    Exposure time: 5 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750863) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"