GALC Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH03-45] (HA750879)
Catalog# HA750879
Rabbit Monoclonal to GALC
Tested for
-
WB
-
IF-Cell
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of GALC on different lysates with Rabbit anti-GALC antibody (HA750879) at 1/5,000 dilution.
Lane 1: 293T cell lysate
Lane 2: A375 cell lysate
Lane 3: A549 cell lysate
Lane 4: HepG2 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: PC-12 cell lysate
Lane 7: Mouse brain tissue lysate
Lane 8: Mouse testis tissue lysate
Lane 9: Rat brain tissue lysate
Lane 10: Rat testis tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 77 kDa
Observed band size: 60 kDa
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750879) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T cell lysate
Lane 2: A375 cell lysate
Lane 3: A549 cell lysate
Lane 4: HepG2 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: PC-12 cell lysate
Lane 7: Mouse brain tissue lysate
Lane 8: Mouse testis tissue lysate
Lane 9: Rat brain tissue lysate
Lane 10: Rat testis tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 77 kDa
Observed band size: 60 kDa
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750879) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.