Plexin B2 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH03-48] (HA750882)
Catalog# HA750882
Rabbit Monoclonal to Plexin B2
Tested for
-
WB
-
IF-Cell
-
IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Plexin B2 on different lysates with Rabbit anti-Plexin B2 antibody (HA750882) at 1/2,000 dilution.
Lane 1: LNCaP cell lysate
Lane 2: 293T cell lysate
Lane 3: HeLa cell lysate
Lane 4: U-87 MG cell lysate
Lane 5: HepG2 cell lysate
Lane 6: K-562 cell lysate
Lane 7: Jurkat cell lysate
Lane 8: MCF7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 205 kDa
Observed band size: 170/240 kDa
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750882) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: LNCaP cell lysate
Lane 2: 293T cell lysate
Lane 3: HeLa cell lysate
Lane 4: U-87 MG cell lysate
Lane 5: HepG2 cell lysate
Lane 6: K-562 cell lysate
Lane 7: Jurkat cell lysate
Lane 8: MCF7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 205 kDa
Observed band size: 170/240 kDa
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750882) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.