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UFSP2 Recombinant Rabbit Monoclonal Antibody [PSH03-57] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750891

UFSP2 Recombinant Rabbit Monoclonal Antibody [PSH03-57] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

UFSP2 Recombinant Rabbit Monoclonal Antibody [PSH03-57] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human UFSP2 aa 420-469 / 469.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 53 kDa

Positive Control

293T cell lysate, HeLa cell lysate, HepG2 cell lysate, U-87 MG cell lysate, Neuro-2a cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, C6 cell lysate, human colon tissue, human stomach tissue, mouse kidney tissue, rat kidney tissue.

Conjugation

unconjugated

Clone Number

PSH03-57

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

UFM1 specific peptidase 2 is a protein that in humans is encoded by the UFSP2 gene. This gene encodes a highly conserved cysteine protease. The protein cleaves two C-terminal residues from ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein. Activation of ubiquitin-fold modifier 1 by the encoded protein exposes a C-terminal glycine residue that allows interaction with other proteins and transfer to its target protein. An allelic variant of this gene has been associated with Beukes hip dysplasia. Alternative splicing results in multiple transcript variants.

Background References

1. Cao Z et al. The UFSP2/UFMylation Pathway Is Involved in Silica-Induced Pulmonary Injury. DNA Cell Biol. 2021 Apr

2. Zhang G et al. UFSP2-related spondyloepimetaphyseal dysplasia: A confirmatory report. Eur J Med Genet. 2020 Nov

Subcellular Location

Cytoplasm, Endoplasmic reticulum, Nucleus.

UNIPROT

SwissProt: Q9NUQ7 Human

SwissProt: Q99K23 Mouse

SwissProt: Q5XIB4 Rat

Synonyms

C4orf20 antibody

Chromosome 4 open reading frame 20 antibody

FLJ11200 antibody

Ufm1 specific protease 2 antibody

UFM1-specific peptidase 2 antibody

Ufm1-specific protease 2 antibody

UfSP2 antibody

UFSP2_HUMAN antibody

Images

  • This data was developed using <a href="/products/HA722016" style="font-weight: bold;text-decoration: underline;">HA722016</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of UFSP2 on different lysates with Rabbit anti-UFSP2 antibody (<a href="/products/HA722016" style="font-weight: bold;text-decoration: underline;">HA722016</a>) at 1/2,000 dilution.<br /><br />Lane 1: 293T cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: HepG2 cell lysate<br />Lane 4: U-87 MG cell lysate<br />Lane 5: Neuro-2a cell lysate<br />Lane 6: RAW264.7 cell lysate<br />Lane 7: PC-12 cell lysate<br />Lane 8: C6 cell lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 53 kDa<br />Observed band size: 53 kDa<br /><br />Exposure time: 1 minute 40 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722016" style="font-weight: bold;text-decoration: underline;">HA722016</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    This data was developed using HA722016, the same antibody clone in a different buffer formulation.
    Western blot analysis of UFSP2 on different lysates with Rabbit anti-UFSP2 antibody (HA722016) at 1/2,000 dilution.

    Lane 1: 293T cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: HepG2 cell lysate
    Lane 4: U-87 MG cell lysate
    Lane 5: Neuro-2a cell lysate
    Lane 6: RAW264.7 cell lysate
    Lane 7: PC-12 cell lysate
    Lane 8: C6 cell lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 53 kDa
    Observed band size: 53 kDa

    Exposure time: 1 minute 40 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722016) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • This data was developed using <a href="/products/HA722016" style="font-weight: bold;text-decoration: underline;">HA722016</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-UFSP2 antibody (<a href="/products/HA722016" style="font-weight: bold;text-decoration: underline;">HA722016</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722016" style="font-weight: bold;text-decoration: underline;">HA722016</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA722016, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-UFSP2 antibody (HA722016) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722016) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA722016" style="font-weight: bold;text-decoration: underline;">HA722016</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-UFSP2 antibody (<a href="/products/HA722016" style="font-weight: bold;text-decoration: underline;">HA722016</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722016" style="font-weight: bold;text-decoration: underline;">HA722016</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA722016, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-UFSP2 antibody (HA722016) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722016) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA722016" style="font-weight: bold;text-decoration: underline;">HA722016</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-UFSP2 antibody (<a href="/products/HA722016" style="font-weight: bold;text-decoration: underline;">HA722016</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722016" style="font-weight: bold;text-decoration: underline;">HA722016</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA722016, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-UFSP2 antibody (HA722016) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722016) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA722016" style="font-weight: bold;text-decoration: underline;">HA722016</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-UFSP2 antibody (<a href="/products/HA722016" style="font-weight: bold;text-decoration: underline;">HA722016</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722016" style="font-weight: bold;text-decoration: underline;">HA722016</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA722016, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-UFSP2 antibody (HA722016) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722016) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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