p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 Recombinant Rabbit Monoclonal Antibody [PSH03-60] - BSA and Azide free
Catalog# HA750894
p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 Recombinant Rabbit Monoclonal Antibody [PSH03-60] - BSA and Azide free
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WB
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Human
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Mouse
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Rat
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Green monkey
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HA722019
含抗保成分
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unconjugated
Overview
Product Name
p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 Recombinant Rabbit Monoclonal Antibody [PSH03-60] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human MAPK12 aa 1-367 / 367.
Product Specificity
Anti-p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 Antibody [PSH03-60] detects endogenous levels of total p38α, p38β and p38γ MAPK protein. This antibody does not recognize p38δ MAPK protein.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB
Molecular Weight
Predicted band size: 42 kDa
Positive Control
293T cell lysate, HeLa cell lysate, K-562 cell lysate, MDA-MB-231 cell lysate, COS-1 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, rat heart tissue lysate.
Conjugation
unconjugated
Clone Number
PSH03-60
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
Target
Function
A mitogen-activated protein kinase (MAPK or MAP kinase) is a type of protein kinase that is specific to the amino acids serine and threonine (i.e., a serine/threonine-specific protein kinase). MAPKs are involved in directing cellular responses to a diverse array of stimuli, such as mitogens, osmotic stress, heat shock and proinflammatory cytokines. They regulate cell functions including proliferation, gene expression, differentiation, mitosis, cell survival, and apoptosis. MAP kinases are found in eukaryotes only, but they are fairly diverse and encountered in all animals, fungi and plants, and even in an array of unicellular eukaryotes. MAPKs belong to the CMGC (CDK/MAPK/GSK3/CLK) kinase group. The closest relatives of MAPKs are the cyclin-dependent kinases (CDKs).
Background References
1. Wang J et al. Pan-cancer analysis supports MAPK12 as a potential prognostic and immunotherapeutic target in multiple tumor types, including in THCA. Oncol Lett. 2022 Oct
2. Yang R et al. Prolactin Regulates Ovine Ovarian Granulosa Cell Apoptosis by Affecting the Expression of MAPK12 Gene. Int J Mol Sci. 2023 Jun
Subcellular Location
Cytoplasm, Nucleus, Mitochondrion.
UNIPROT
Synonyms
ERK 6 antibody
ERK-6 antibody
ERK6 antibody
Extracellular signal-regulated kinase 6 antibody
MAP kinase 12 antibody
MAP kinase p38 gamma antibody
MAPK 12 antibody
Mapk12 antibody
Mitogen Activated Protein Kinase 12 antibody
Mitogen activated protein kinase p38 gamma antibody
ExpandImages
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Western blot analysis of p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 on different lysates with Rabbit anti-p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 antibody (HA750894) at 1/2,000 dilution.
Lane 1: 293T cell lysate (30 µg/Lane)
Lane 2: HeLa cell lysate (30 µg/Lane)
Lane 3: K-562 cell lysate (30 µg/Lane)
Lane 4: MDA-MB-231 cell lysate (30 µg/Lane)
Lane 5: COS-1 cell lysate (30 µg/Lane)
Lane 6: Neuro-2a cell lysate (30 µg/Lane)
Lane 7: PC-12 cell lysate (30 µg/Lane)
Lane 8: Mouse skeletal muscle tissue lysate (30 µg/Lane)
Lane 9: Rat skeletal muscle tissue lysate (30 µg/Lane)
Lane 10: Rat heart tissue lysate (30 µg/Lane)
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 40 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750894) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 with Rabbit anti-p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 antibody (HA750894) at 1/2,000 dilution.
Lane 1: Human p38 gamma recombinant protein, 30ng/Lane
Lane 2: Human p38 alpha recombinant protein, 50ng/Lane
Lane 3: Human p38 delta recombinant protein, 50ng/Lane
Lane 4: Human p38 beta recombinant protein, 50ng/Lane
Exposure time: 43 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750894) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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