p38 MAPK Recombinant Rabbit Multiclonal Antibody [PSH03-72] - BSA and Azide free
Catalog# HA750906
p38 MAPK Recombinant Rabbit Multiclonal Antibody [PSH03-72] - BSA and Azide free
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WB
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Human
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Mouse
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Rat
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Green monkey
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unconjugated
Overview
Product Name
p38 MAPK Recombinant Rabbit Multiclonal Antibody [PSH03-72] - BSA and Azide free
Antibody Type
Recombinant Rabbit multiclonal
Immunogen
Recombinant protein within human MAPK12 aa 1-367 / 367.
Product Specificity
Anti-p38 MAPK Antibody [PSH03-72] detects endogenous levels of total p38α, p38β, p38γ and p38δ MAPK protein.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB
Target Molecular Weight
Predicted band size: 42 kDa
Positive Control
293T cell lysate, HeLa cell lysate, K-562 cell lysate, COS-1 cell lysate, PC-12 cell lysate.
Conjugation
unconjugated
Clone Number
PSH03-72
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
A mitogen-activated protein kinase (MAPK or MAP kinase) is a type of protein kinase that is specific to the amino acids serine and threonine (i.e., a serine/threonine-specific protein kinase). MAPKs are involved in directing cellular responses to a diverse array of stimuli, such as mitogens, osmotic stress, heat shock and proinflammatory cytokines. They regulate cell functions including proliferation, gene expression, differentiation, mitosis, cell survival, and apoptosis. MAP kinases are found in eukaryotes only, but they are fairly diverse and encountered in all animals, fungi and plants, and even in an array of unicellular eukaryotes. MAPKs belong to the CMGC (CDK/MAPK/GSK3/CLK) kinase group. The closest relatives of MAPKs are the cyclin-dependent kinases (CDKs). p38 MAP Kinase Antibody detects endogenous levels of total p38 alpha, p38 beta, p38 gamma, p38 delta protein.
Background References
1. Wang J et al. Pan-cancer analysis supports MAPK12 as a potential prognostic and immunotherapeutic target in multiple tumor types, including in THCA. Oncol Lett. 2022 Oct
2. Yang R et al. Prolactin Regulates Ovine Ovarian Granulosa Cell Apoptosis by Affecting the Expression of MAPK12 Gene. Int J Mol Sci. 2023 Jun
Subcellular Location
Cytoplasm, Nucleus, Mitochondrion.
UNIPROT
Synonyms
ERK 6 antibody
ERK-6 antibody
ERK6 antibody
Extracellular signal-regulated kinase 6 antibody
MAP kinase 12 antibody
MAP kinase p38 gamma antibody
MAPK 12 antibody
Mapk12 antibody
Mitogen Activated Protein Kinase 12 antibody
Mitogen activated protein kinase p38 gamma antibody
ExpandImages
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Western blot analysis of p38 MAPK on different lysates with Rabbit anti-p38 MAPK antibody (HA750906) at 1/1,000 dilution.
Lane 1: 293T cell lysate
Lane 2: HeLa cell lysate
Lane 3: K-562 cell lysate
Lane 4: COS-1 cell lysate
Lane 5: PC-12 cell lysate
Lysates/proteins at 30 µg/Lane.
Exposure time: 4seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA750906, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 42 kDa
Observed band size: 42 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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