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CRLF3 Recombinant Rabbit Monoclonal Antibody [PSH03-88] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750913

CRLF3 Recombinant Rabbit Monoclonal Antibody [PSH03-88] - BSA and Azide free

Application

  • WB

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

CRLF3 Recombinant Rabbit Monoclonal Antibody [PSH03-88] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human CRLF3 aa 1-442 / 442.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell

Molecular Weight

Predicted band size: 50 kDa

Positive Control

HeLa cell lysate, Raji cell lysate, Ramos cell lysate, K-562 cell lysate, Jurkat cell lysate, 293T cell lysate, A549 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, mouse thymus tissue lysate, mouse spleen tissue lysate, rat thymus tissue lysate, rat testis tissue lysate, RAW264.7.

Conjugation

unconjugated

Clone Number

PSH03-88

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IF-Cell

  • 1:100

Target

Function

Cytokine receptor-like factor 3 is a protein that in humans is encoded by the CRLF3 gene. Model organisms have been used in the study of CRLF3 function. A conditional knockout mouse line, called Crlf3tm1a(KOMP)Wtsi was generated as part of the International Knockout Mouse Consortium program, a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists. Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion. Twenty six tests were carried out and two significant phenotypes were reported. Homozygous mutant female adults had a significant increase in circulating levels of fructosamine, while mutants of both sexes had decreased platelet cell numbers. Although CRLF3 signaling pathways have not yet been fully characterized it is very likely that CRLF3 is a neuroprotective erythropoietin receptor.

Background References

1. Taznin T et al. Cytokine Receptor-Like Factor 3 (CRLF3) Contributes to Early Zebrafish Hematopoiesis. Front Immunol. 2022 Jun

2. Bennett C et al. CRLF3 plays a key role in the final stage of platelet genesis and is a potential therapeutic target for thrombocythemia. Blood. 2022 Apr

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: Q8IUI8 Human

SwissProt: Q9Z2L7 Mouse

Entrez Gene: 54395 Rat

Synonyms

CREME 9 antibody

CREME9 antibody

CRLF 3 antibody

CRLM9 antibody

Cytokine receptor like factor 3 antibody

Cytokine receptor like molecule 9 antibody

Cytokine receptor related protein 4 antibody

CYTOR4 antibody

FRWS antibody

MGC20661 antibody

Expand

Images

  • Western blot analysis of CRLF3 on different lysates with Rabbit anti-CRLF3 antibody (<a href="/products/HA750913" style="font-weight: bold;text-decoration: underline;">HA750913</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: Raji cell lysate (20 µg/Lane)<br />Lane 3: Ramos cell lysate (20 µg/Lane)<br />Lane 4: K-562 cell lysate (20 µg/Lane)<br />Lane 5: Jurkat cell lysate (20 µg/Lane)<br />Lane 6: 293T cell lysate (20 µg/Lane)<br />Lane 7: A549 cell lysate (20 µg/Lane)<br />Lane 8: RAW264.7 cell lysate (20 µg/Lane)<br />Lane 9: PC-12 cell lysate (20 µg/Lane)<br />Lane 10: Mouse thymus tissue lysate (40 µg/Lane)<br />Lane 11: Mouse spleen tissue lysate (40 µg/Lane)<br />Lane 12: Rat thymus tissue lysate (40 µg/Lane)<br />Lane 13: Rat testis tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 50 kDa<br />Observed band size: 50 kDa<br />Exposure time: 30 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750913" style="font-weight: bold;text-decoration: underline;">HA750913</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CRLF3 on different lysates with Rabbit anti-CRLF3 antibody (HA750913) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: Raji cell lysate (20 µg/Lane)
    Lane 3: Ramos cell lysate (20 µg/Lane)
    Lane 4: K-562 cell lysate (20 µg/Lane)
    Lane 5: Jurkat cell lysate (20 µg/Lane)
    Lane 6: 293T cell lysate (20 µg/Lane)
    Lane 7: A549 cell lysate (20 µg/Lane)
    Lane 8: RAW264.7 cell lysate (20 µg/Lane)
    Lane 9: PC-12 cell lysate (20 µg/Lane)
    Lane 10: Mouse thymus tissue lysate (40 µg/Lane)
    Lane 11: Mouse spleen tissue lysate (40 µg/Lane)
    Lane 12: Rat thymus tissue lysate (40 µg/Lane)
    Lane 13: Rat testis tissue lysate (40 µg/Lane)

    Predicted band size: 50 kDa
    Observed band size: 50 kDa
    Exposure time: 30 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750913) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of RAW264.7 cells labeling CRLF3 with Rabbit anti-CRLF3 antibody (<a href="/products/HA750913" style="font-weight: bold;text-decoration: underline;">HA750913</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CRLF3 antibody (<a href="/products/HA750913" style="font-weight: bold;text-decoration: underline;">HA750913</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of RAW264.7 cells labeling CRLF3 with Rabbit anti-CRLF3 antibody (HA750913) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CRLF3 antibody (HA750913) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"