CRLF3 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH03-88] (HA750913)
Catalog# HA750913
Rabbit Monoclonal to CRLF3
Tested for
-
WB
-
IF-Cell
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of CRLF3 on different lysates with Rabbit anti-CRLF3 antibody (HA750913) at 1/2,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Raji cell lysate (20 µg/Lane)
Lane 3: Ramos cell lysate (20 µg/Lane)
Lane 4: K-562 cell lysate (20 µg/Lane)
Lane 5: Jurkat cell lysate (20 µg/Lane)
Lane 6: 293T cell lysate (20 µg/Lane)
Lane 7: A549 cell lysate (20 µg/Lane)
Lane 8: RAW264.7 cell lysate (20 µg/Lane)
Lane 9: PC-12 cell lysate (20 µg/Lane)
Lane 10: Mouse thymus tissue lysate (40 µg/Lane)
Lane 11: Mouse spleen tissue lysate (40 µg/Lane)
Lane 12: Rat thymus tissue lysate (40 µg/Lane)
Lane 13: Rat testis tissue lysate (40 µg/Lane)
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750913) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Raji cell lysate (20 µg/Lane)
Lane 3: Ramos cell lysate (20 µg/Lane)
Lane 4: K-562 cell lysate (20 µg/Lane)
Lane 5: Jurkat cell lysate (20 µg/Lane)
Lane 6: 293T cell lysate (20 µg/Lane)
Lane 7: A549 cell lysate (20 µg/Lane)
Lane 8: RAW264.7 cell lysate (20 µg/Lane)
Lane 9: PC-12 cell lysate (20 µg/Lane)
Lane 10: Mouse thymus tissue lysate (40 µg/Lane)
Lane 11: Mouse spleen tissue lysate (40 µg/Lane)
Lane 12: Rat thymus tissue lysate (40 µg/Lane)
Lane 13: Rat testis tissue lysate (40 µg/Lane)
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750913) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.