KDM5B Recombinant Rabbit Monoclonal Antibody [PSH03-97] - BSA and Azide free
Catalog# HA750922
KDM5B Recombinant Rabbit Monoclonal Antibody [PSH03-97] - BSA and Azide free
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WB
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IP
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Human
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HA722076
含抗保成分
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unconjugated
Overview
Product Name
KDM5B Recombinant Rabbit Monoclonal Antibody [PSH03-97] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human KDM5B aa 960-1,009 / 1,544.
Species Reactivity
Human
Validated Applications
WB, IP
Molecular Weight
Predicted band size: 176 kDa
Positive Control
BT-20 cell lysate, MDA-MB-468 cell lysate, AGS cell lysate, A549 cell lysate.
Conjugation
unconjugated
Clone Number
PSH03-97
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IP
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1-2μg/sample
Target
Function
PLU-1 is a large (1,544 amino acid) nuclear protein that interacts with brain factor-1 (BF-1) and paired box 9 (PAX9), both of which are developmental transcription factors. PLU-1 belongs to the testis-cancer antigen group of proteins and is a member of the ARID family of DNA binding proteins. It is a multi-domain protein with strong transcriptional repression properties. PLU-1 shows restricted expression in adult tissues, with high expression in testis and transient expression in the pregnant mammary gland. Both the PLU-1 gene and the PLU-1 protein product are specifically upregulated in breast cancer. PLU-1 may be important in meiotic transcription because of its apparent association with chromatin.
Background References
1. Tan K et al. Human PLU-1 has transcriptional repression properties and interacts with the developmental transcription factors BF-1 and PAX9. J Biol Chem 278:20507-20513 (2003).
2. Roesch A et al. Re-expression of the retinoblastoma-binding protein 2-homolog 1 reveals tumor-suppressive functions in highly metastatic melanoma cells. J Invest Dermatol 126:1850-1859 (2006).
Subcellular Location
Nucleus.
UNIPROT
Synonyms
Cancer/testis antigen 31 antibody
CT31 antibody
Histone demethylase JARID1B antibody
JARID1B antibody
Jumonji AT rich interactive domain 1B antibody
Jumonji/ARID domain-containing protein 1B antibody
Kdm5b antibody
KDM5B_HUMAN antibody
Lysine (K) specific demethylase 5B antibody
Lysine demethylase 5B antibody
ExpandImages
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Western blot analysis of KDM5B on different lysates with Rabbit anti-KDM5B antibody (HA750922) at 1/2,000 dilution.
Lane 1: BT-20 cell lysate
Lane 2: MDA-MB-468 cell lysate
Lane 3: AGS cell lysate
Lane 4: A549 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 176 kDa
Observed band size: 145 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750922) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/200,000 dilution was used for 1 hour at room temperature. -
KDM5B was immunoprecipitated in 0.2mg A549 cell lysate with HA750922 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750922 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: A549 cell lysate (input)
Lane 2: ER1802-21 IP in A549 cell lysate
Lane 3: Rabbit IgG instead of ER1802-21 in A549 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 24 seconds
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"