Complement C5 Recombinant Rabbit Monoclonal Antibody [PSH04-01] - BSA and Azide free
Catalog# HA750924
Complement C5 Recombinant Rabbit Monoclonal Antibody [PSH04-01] - BSA and Azide free
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WB
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IF-Cell
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IHC-P
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FC
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Human
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unconjugated
Overview
Product Name
Complement C5 Recombinant Rabbit Monoclonal Antibody [PSH04-01] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human C5 aa 1-900 / 1,676.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IHC-P, FC
Target Molecular Weight
Predicted band size: 188 kDa
Positive Control
HepG2 cell lysate, human liver tissue lysate, human plasma lysates, HepG2, human breast tissue, human liver tissue.
Conjugation
unconjugated
Clone Number
PSH04-01
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Complement component 5 is a protein that in humans is encoded by the C5 gene. Complement component 5 is involved in the complement system. It is cleaved into C5a and C5b: C5a plays an important role in chemotaxis; C5b forms the first part of the complement membrane attack complex. Deficiency is thought to cause Leiner's disease. Complement component 5 is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is composed of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a C5-convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components.
Background References
1. Röth A et al. The complement C5 inhibitor crovalimab in paroxysmal nocturnal hemoglobinuria. Blood. 2020 Mar
2. Li Z et al. Glioblastoma Cell-Derived lncRNA-Containing Exosomes Induce Microglia to Produce Complement C5, Promoting Chemotherapy Resistance. Cancer Immunol Res. 2021 Dec
Subcellular Location
Secreted.
UNIPROT
Synonyms
Complement C5
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
Complement C5 beta chain
Complement C5 alpha chain
C5a anaphylatoxin
Complement C5 alpha' chain
C5
CPAMD4
Images
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This data was developed using HA722082, the same antibody clone in a different buffer formulation.
Western blot analysis of Complement C5 on different lysates with Rabbit anti-Complement C5 antibody (HA722082) at 1/2,000 dilution.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: Human liver tissue lysate (40 µg/Lane)
Predicted band size: 188 kDa
Observed band size: 188/72 kDa
Exposure time: 43 seconds;
ECL: K1802
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722082) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA722082, the same antibody clone in a different buffer formulation.
Western blot analysis of Complement C5 on human plasma lysates with Rabbit anti-Complement C5 antibody (HA722082) at 1/2,000 dilution.
Lysates/proteins at 40 µg/Lane.
Predicted band size: 188 kDa
Observed band size: 72 kDa
Exposure time: 43 seconds;
ECL: K1801
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722082) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA722082, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of HepG2 cells labeling Complement C5 with Rabbit anti-Complement C5 antibody (HA722082) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Complement C5 antibody (HA722082) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
This data was developed using HA722082, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Complement C5 antibody (HA722082) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722082) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA722082, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Complement C5 antibody (HA722082) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722082) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA722082, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of HepG2 cells labeling Complement C5.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA722082, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"