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CTU2 Recombinant Rabbit Monoclonal Antibody [PSH04-05] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750928

CTU2 Recombinant Rabbit Monoclonal Antibody [PSH04-05] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Green monkey

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

CTU2 Recombinant Rabbit Monoclonal Antibody [PSH04-05] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human CTU2 aa 1-515 / 515.

Species Reactivity

Human, Mouse, Green monkey

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 56 kDa

Positive Control

HeLa cell lysate, HT-29 cell lysate, 293T cell lysate, K-562 cell lysate, Raji cell lysate, HepG2 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, mouse spleen tissue lysate, mouse liver tissue.

Conjugation

unconjugated

Clone Number

PSH04-05

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:200

Target

Function

CTU2 (formerly known as C16orf84) is a human gene located on chromosome 16.[5] The mRNA encodes the longer isoform. The gene encodes a cytoplasmic protein that plays a probable role in tRNA modification.

Background References

1. Shaheen R et al. Biallelic variants in CTU2 cause DREAM-PL syndrome and impair thiolation of tRNA wobble U34. Hum Mutat. 2019 Nov

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: Q2VPK5 Human

SwissProt: Q3U308 Mouse

Synonyms

C16orf84 antibody

ctu2 antibody

CTU2_HUMAN antibody

Cytoplasmic tRNA 2-thiolation protein 2 antibody

cytosolic thiouridylase subunit 2 homolog (S. pombe) antibody

NCS2 antibody

PF0432 antibody

Images

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-CTU2 antibody (<a href="/products/HA750928" style="font-weight: bold;text-decoration: underline;">HA750928</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750928" style="font-weight: bold;text-decoration: underline;">HA750928</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-CTU2 antibody (HA750928) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750928) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of CTU2 on different lysates with Rabbit anti-CTU2 antibody (<a href="/products/HA750928" style="font-weight: bold;text-decoration: underline;">HA750928</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HT-29 cell lysate<br />Lane 3: 293T cell lysate<br />Lane 4: K-562 cell lysate<br />Lane 5: Raji cell lysate<br />Lane 6: HepG2 cell lysate<br />Lane 7: COS-1 cell lysate<br />Lane 8: NIH/3T3 cell lysate<br />Lane 9: Mouse spleen tissue lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br />Exposure time: 24 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA750928" style="font-weight: bold;text-decoration: underline;">HA750928</a>, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 56 kDa<br />Observed band size: 56 kDa

    Western blot analysis of CTU2 on different lysates with Rabbit anti-CTU2 antibody (HA750928) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HT-29 cell lysate
    Lane 3: 293T cell lysate
    Lane 4: K-562 cell lysate
    Lane 5: Raji cell lysate
    Lane 6: HepG2 cell lysate
    Lane 7: COS-1 cell lysate
    Lane 8: NIH/3T3 cell lysate
    Lane 9: Mouse spleen tissue lysate

    Lysates/proteins at 30 µg/Lane.
    Exposure time: 24 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA750928, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 56 kDa
    Observed band size: 56 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"