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ROR gamma T Recombinant Rabbit Monoclonal Antibody [PSH04-38] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750947

ROR gamma T Recombinant Rabbit Monoclonal Antibody [PSH04-38] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

ROR gamma T Recombinant Rabbit Monoclonal Antibody [PSH04-38] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human ROR gamma T aa 101-300 / 497 (P51449-2).

Species Reactivity

Human, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 58 kDa

Positive Control

HCT 116 cell lysate, rat thymus tissue lysate, human thymus tissue, rat thymus tissue.

Conjugation

unconjugated

Clone Number

PSH04-38

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:500

Target

Function

RAR-related orphan receptor gamma (RORγ) is a protein that in humans is encoded by the RORC (RAR-related orphan receptor C) gene. RORγ is a member of the nuclear receptor family of transcription factors. It is mainly expressed in immune cells (Th17 cells) and it also regulates circadian rhythms. It may be involved in the progression of certain types of cancer. Two isoforms are produced from the same RORC gene, probably by selection of alternative promoters. RORγ (also referred to as RORγ1) – produced from an mRNA containing exons 1 to 11. RORγt (also known as RORγ2) – produced from an mRNA identical to that of RORγ, except that the two 5'-most exons are replaced by an alternative exon, located downstream in the gene. This causes a different, shorter N-terminus.

Background References

1. Kedmi R et al. A RORγt+ cell instructs gut microbiota-specific Treg cell differentiation. Nature. 2022 Oct

2. Hanna BS et al. The gut microbiota promotes distal tissue regeneration via RORγ+ regulatory T cell emissaries. Immunity. 2023 Apr

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P51449 Human

Entrez Gene: 368158 Rat

Synonyms

IMD42 antibody

MGC129539 antibody

NR1F3 antibody

Nuclear receptor ROR gamma antibody

Nuclear receptor ROR-gamma antibody

Nuclear receptor RZR gamma antibody

Nuclear receptor RZR-gamma antibody

Nuclear receptor subfamily 1 group F member 3 antibody

RAR related orphan nuclear receptor variant 2 antibody

RAR related orphan receptor C antibody

Expand

Images

  • Western blot analysis of ROR gamma T on different lysates with Rabbit anti-ROR gamma T antibody (<a href="/products/HA750947" style="font-weight: bold;text-decoration: underline;">HA750947</a>) at 1/2,000 dilution.<br /><br />Lane 1: HCT 116 cell lysate<br />Lane 2: Rat thymus tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 58 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750947" style="font-weight: bold;text-decoration: underline;">HA750947</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ROR gamma T on different lysates with Rabbit anti-ROR gamma T antibody (HA750947) at 1/2,000 dilution.

    Lane 1: HCT 116 cell lysate
    Lane 2: Rat thymus tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 58 kDa
    Observed band size: 55 kDa

    Exposure time: 3 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750947) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human thymus tissue with Rabbit anti-ROR gamma T antibody (<a href="/products/HA750947" style="font-weight: bold;text-decoration: underline;">HA750947</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750947" style="font-weight: bold;text-decoration: underline;">HA750947</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human thymus tissue with Rabbit anti-ROR gamma T antibody (HA750947) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750947) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat thymus tissue with Rabbit anti-ROR gamma T antibody (<a href="/products/HA750947" style="font-weight: bold;text-decoration: underline;">HA750947</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750947" style="font-weight: bold;text-decoration: underline;">HA750947</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat thymus tissue with Rabbit anti-ROR gamma T antibody (HA750947) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750947) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"