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Digoxigenin Recombinant Rabbit Monoclonal Antibody [PSH04-39] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750948

Digoxigenin Recombinant Rabbit Monoclonal Antibody [PSH04-39] - BSA and Azide free

Application

  • WB

  • IHC-P

  • IHC-Fr

  • Dot Blot

  • ELISA

  • IF-Tissue

  • IP

Reactivity

  • Species independent

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Digoxigenin Recombinant Rabbit Monoclonal Antibody [PSH04-39] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

DIG-OVA

Species Reactivity

Species independent

Validated Applications

WB, IHC-P, IHC-Fr, Dot Blot, ELISA, IF-Tissue, IP

Conjugation

unconjugated

Clone Number

PSH04-39

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:200-1:5,000

  • IHC-Fr

  • 1:50-1:1,000

  • Dot Blot

  • 1:2,000

  • ELISA

  • 1:5,000-1:20,000

  • IF-Tissue

  • 1:50-1:1,000

  • IP

  • 1-2μg/sample

Target

Function

Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea, Digitalis orientalis and Digitalis lanata (foxgloves), where it is attached to sugars, to form the glycosides (e.g. digoxin, lanatoside C). Digoxigenin is a hapten, a small molecule with high antigenicity, that is used in many molecular biology applications similarly to other popular haptens such as 2,4-Dinitrophenol, biotin, and fluorescein. Typically, digoxigenin is introduced chemically (conjugation) into biomolecules (proteins, nucleic acids) to be detected in further assays. Kd of the digoxigenin-antibody interaction has been estimated at ~12 nM (compare to Kd~0.1pM for the biotin-streptavidin interaction).

Background References

1. Barratt KS et al. Production of Digoxigenin-Labeled Riboprobes for In Situ Hybridization Experiments. Curr Protoc Mouse Biol. 2020 Jun

2. Tanji M et al. Digoxigenin-labeled RNA probes for untranslated regions enable the isoform-specific gene expression analysis of myosin heavy chains in whole-mount in situ hybridization. Dev Growth Differ. 2023 Jan

Synonyms

1672-46-4 antibody

BRN 0096479 antibody

DIG antibody

EINECS 216-806-2 antibody

HSDB 7108 antibody

Lanadigenin antibody

Lanadigigenin antibody

ST056392 antibody

Digoxigenin

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Digoxigenin on different lysates with Rabbit anti-Digoxigenin antibody (<a href="/products/HA750948" style="font-weight: bold;text-decoration: underline;">HA750948</a>) at 1/2,000 dilution.<br /><br />Lane 1: DIG-BSA (2 ng/Lane)<br />Lane 2: BSA (negative) (2 ng/Lane)<br />Lane 3: 293T cell lysate (negative) (20 µg/Lane)<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750948" style="font-weight: bold;text-decoration: underline;">HA750948</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Digoxigenin on different lysates with Rabbit anti-Digoxigenin antibody (HA750948) at 1/2,000 dilution.

    Lane 1: DIG-BSA (2 ng/Lane)
    Lane 2: BSA (negative) (2 ng/Lane)
    Lane 3: 293T cell lysate (negative) (20 µg/Lane)

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750948) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded human tonsil tissue (negative) with Rabbit anti-Digoxigenin antibody (<a href="/products/HA750948" style="font-weight: bold;text-decoration: underline;">HA750948</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750948" style="font-weight: bold;text-decoration: underline;">HA750948</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue (negative) with Rabbit anti-Digoxigenin antibody (HA750948) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750948) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded mouse spleen tissue (negative) with Rabbit anti-Digoxigenin antibody (<a href="/products/HA750948" style="font-weight: bold;text-decoration: underline;">HA750948</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750948" style="font-weight: bold;text-decoration: underline;">HA750948</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue (negative) with Rabbit anti-Digoxigenin antibody (HA750948) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750948) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"