TIMP3 Recombinant Rabbit Monoclonal Antibody [PSH04-41] - BSA and Azide free
Catalog# HA750950
TIMP3 Recombinant Rabbit Monoclonal Antibody [PSH04-41] - BSA and Azide free
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WB
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IF-Cell
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Human
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Rat
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unconjugated
Overview
Product Name
TIMP3 Recombinant Rabbit Monoclonal Antibody [PSH04-41] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human TIMP3 aa 1-211 / 211.
Species Reactivity
Human, Rat
Validated Applications
WB, IF-Cell
Target Molecular Weight
Predicted band size: 24 kDa
Positive Control
HepG2 cell lysate, A431 cell lysate, rat placenta tissue lysate, rat spleen tissue lysate, HeLa, HepG2.
Conjugation
unconjugated
Clone Number
PSH04-41
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Metalloproteinase inhibitor 3 is a protein that in humans is encoded by the TIMP3 gene. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy.
Background References
1. Casagrande V et al. TIMP3 involvement and potentiality in the diagnosis, prognosis and treatment of diabetic nephropathy. Acta Diabetol. 2021 Dec
2. Guan B et al. Early-Onset TIMP3-Related Retinopathy Associated With Impaired Signal Peptide. JAMA Ophthalmol. 2022 Jul
Subcellular Location
Secreted, extracellular space, extracellular matrix.
Synonyms
HSMRK222 antibody
K222 antibody
K222TA2 antibody
Metalloproteinase inhibitor 3 antibody
MIG 5 protein antibody
MIG5 protein antibody
Protein MIG 5 antibody
Protein MIG-5 antibody
SFD antibody
Sorsby fundus dystrophy pseudoinflammatory antibody
ExpandImages
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☑ Relative expression (RE)
This data was developed using HA722126, the same antibody clone in a different buffer formulation.
Western blot analysis of TIMP3 on different lysates with Rabbit anti-TIMP3 antibody (HA722126) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: A431 cell lysate
Lane 3: Human liver tissue lysate (negative)
Lane 4: Rat placenta tissue lysate
Lane 5: Rat spleen tissue lysate
Lane 6: Rat liver tissue lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 24 kDa
Observed band size: 20 kDa
Exposure time: 46 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722126) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA722126, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of HeLa cells labeling TIMP3 with Rabbit anti-TIMP3 antibody (HA722126) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TIMP3 antibody (HA722126) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
This data was developed using HA722126, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of HepG2 cells labeling TIMP3 with Rabbit anti-TIMP3 antibody (HA722126) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TIMP3 antibody (HA722126) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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