Ly6c Recombinant Rabbit Monoclonal Antibody [PSH04-51] - BSA and Azide free
Catalog# HA750958
Ly6c Recombinant Rabbit Monoclonal Antibody [PSH04-51] - BSA and Azide free
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WB
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Mouse
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unconjugated
Overview
Product Name
Ly6c Recombinant Rabbit Monoclonal Antibody [PSH04-51] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within mouse Ly6c1 aa 51-100 / 131.
Species Reactivity
Mouse
Validated Applications
WB
Target Molecular Weight
Predicted band size: 14 kDa
Positive Control
Mouse lung tissue lysate, mouse kidney tissue lysate, mouse heart tissue lysate.
Conjugation
unconjugated
Clone Number
PSH04-51
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
The gene encoding the mouse alloantigen, Ly-6C, maps to chromosome 15 and encodes a 131 amino acid protein that belongs to the Ly-6 family of glycosyl-phosphatidylinositol (GPI)-linked proteins. Ly-6 family members share amino acid homology throughout a distinctive cysteine rich protein domain that incorporates O-linked carbohydrates. Murine Ly-6 molecules have unique patterns of tissue expression during hematopoiesis from multipotential stem cells to lineage committed precursor cells, and on specific leukocyte subpopulations in the peripheral lymphoid tissues. Ly-6C is predominantly expressed on murine peripheral CD8 T cells. Ly-6C is involved in endothelial adhesion, the killing of target cells by CTLs, inducing TCR-mediated activation of IL-2 and IFN-γ production in CD8 T cells and the homing of CD8 T cells. In addition, Ly-6C may act as a signaling molecule of LFA-1 activation.
Background References
1. Liu Z et al. Dendritic cell type 3 arises from Ly6C+ monocyte-dendritic cell progenitors. Immunity. 2023 Aug
2. Zhang S et al. CD11b + CD43 hi Ly6C lo splenocyte-derived macrophages exacerbate liver fibrosis via spleen-liver axis. Hepatology. 2023 May
Subcellular Location
Cell membrane.
Synonyms
Ly 6c antibody
Ly6c protein antibody
Ly6c1 antibody
Ly6c2 antibody
Lymphocyte antigen 6 complex antibody
Lymphocyte antigen 6 complex locus C antibody
Lymphocyte antigen 6C antibody
Lymphocyte antigen 6C1 antibody
Lymphocyte antigen 6C2 antibody
Lymphocyte antigen Ly 6C antibody
Images
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☑ Relative expression (RE)
This data was developed using HA722134, the same antibody clone in a different buffer formulation.
Western blot analysis of Ly6c on different lysates with Rabbit anti-Ly6c antibody (HA722134) at 1/1,000 dilution.
Lane 1: Mouse lung tissue lysate
Lane 2: Mouse testis tissue lysate (low expression)
Lane 3: Mouse liver tissue lysate (low expression)
Lane 4: Mouse kidney tissue lysate
Lane 5: Mouse heart tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722134) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"