AGL/Alpha-glucosidase Recombinant Rabbit Monoclonal Antibody [PSH04-56] - BSA and Azide free
Catalog# HA750963
AGL/Alpha-glucosidase Recombinant Rabbit Monoclonal Antibody [PSH04-56] - BSA and Azide free
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WB
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IHC-P
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Human
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Mouse
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Rat
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HA722155
含抗保成分
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unconjugated
Overview
Product Name
AGL/Alpha-glucosidase Recombinant Rabbit Monoclonal Antibody [PSH04-56] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human AGL aa 783-1,532 / 1,532.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 175 kDa
Positive Control
K-562 cell lysate, HEK-293 cell lysate, mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, human liver tissue, mouse liver tissue.
Conjugation
unconjugated
Clone Number
PSH04-56
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:1,000
Target
Function
The glycogen debranching enzyme, in humans, is the protein encoded by the gene AGL. This enzyme is essential for the breakdown of glycogen, which serves as a store of glucose in the body. It has separate glucosyltransferase and glucosidase activities. Together with phosphorylases, the enzyme mobilize glucose reserves from glycogen deposits in the muscles and liver. This constitutes a major source of energy reserves in most organisms. Glycogen breakdown is highly regulated in the body, especially in the liver, by various hormones including insulin and glucagon, to maintain a homeostatic balance of blood-glucose levels. When glycogen breakdown is compromised by mutations in the glycogen debranching enzyme, metabolic diseases such as Glycogen storage disease type III can result. The two steps of glycogen breakdown, glucosyltransferase and glucosidase, are performed by a single enzyme in mammals, yeast, and some bacteria, but by two distinct enzymes in E. coli and other bacteria, complicating nomenclature. Proteins that catalyze both functions are referred to as glycogen debranching enzymes (GDEs). When glucosyltransferase and glucosidase are catalyzed by distinct enzymes, glycogen debranching enzyme usually refers to the glucosidase enzyme. In some literature, an enzyme capable only of glucosidase is referred to as a debranching enzyme.
Background References
1. Ikeda A et al. Glycogen debranching pathway deduced from substrate specificity of glycogen debranching enzyme. Glycoconj J. 2022 Jun
2. Shen M et al. Crystal structures of glycogen-debranching enzyme mutants in complex with oligosaccharides. Acta Crystallogr F Struct Biol Commun. 2021 Nov
Subcellular Location
Cytoplasm.
Synonyms
1110061O17Rik antibody
4-1 antibody
4-glucantransferase antibody
6-glucosidase antibody
9430004C13Rik antibody
9630046L06Rik antibody
AGL antibody
AI850929 antibody
Amylo 1 6 glucosidase 4 alpha glucanotransferase antibody
Amylo-1 antibody
ExpandImages
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Western blot analysis of AGL/Alpha-glucosidase on different lysates with Rabbit anti-AGL/Alpha-glucosidase antibody (HA750963) at 1/1,000 dilution.
Lane 1: K-562 cell lysate (20 µg/Lane)
Lane 2: HEK-293 cell lysate (20 µg/Lane)
Lane 3: Mouse skeletal muscle tissue lysate (10 µg/Lane)
Lane 4: Rat skeletal muscle tissue lysate (10 µg/Lane)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 175 kDa
Observed band size: 150 kDa
Exposure time: Lane 1-2: 3 minutes; Lane 3-4: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750963) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-AGL/Alpha-glucosidase antibody (HA750963) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750963) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-AGL/Alpha-glucosidase antibody (HA750963) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750963) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"