Acid phosphatase/ACP1 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH04-57] (HA750964)
Catalog# HA750964
Rabbit Monoclonal to Acid phosphatase/ACP1
Tested for
-
WB
-
IHC-P
Reacts with
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Human
-
Green monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Acid phosphatase/ACP1 on different lysates with Rabbit anti-Acid phosphatase/ACP1 antibody (HA750964) at 1/2,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: HeLa cell lysate
Lane 3: Jurkat cell lysate
Lane 4: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 18 kDa
Observed band size: 18 kDa
Exposure time: 1 minute 33 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750964) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HepG2 cell lysate
Lane 2: HeLa cell lysate
Lane 3: Jurkat cell lysate
Lane 4: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 18 kDa
Observed band size: 18 kDa
Exposure time: 1 minute 33 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750964) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.