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PD-L1 Recombinant Rabbit Monoclonal Antibody [PSH04-80] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750974

PD-L1 Recombinant Rabbit Monoclonal Antibody [PSH04-80] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Mouse

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

PD-L1 Recombinant Rabbit Monoclonal Antibody [PSH04-80] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse PD-L1 aa 1-250 / 290.

Species Reactivity

Mouse

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 33 kDa

Positive Control

RAW264.7 treated with 10μg/mL LPS for 8 hours cell lysate, J774A.1 treated with 1μg/mL LPS for 24 hours cell lysate, mouse spleen tissue lysate, mouse thymus tissue, mouse lung tissue.

Conjugation

unconjugated

Clone Number

PSH04-80

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:200

Target

Function

Programmed death-ligand 1 (PD-L1) also known as cluster of differentiation 274 (CD274) or B7 homolog 1 (B7-H1) is a protein that in humans is encoded by the CD274 gene. Programmed death-ligand 1 (PD-L1) is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the adaptive arm of immune systems during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. Normally the adaptive immune system reacts to antigens that are associated with immune system activation by exogenous or endogenous danger signals. In turn, clonal expansion of antigen-specific CD8+ T cells and/or CD4+ helper cells is propagated. The binding of PD-L1 to the inhibitory checkpoint molecule PD-1 transmits an inhibitory signal based on interaction with phosphatases (SHP-1 or SHP-2) via Immunoreceptor Tyrosine-Based Switch Motif (ITSM). This reduces the proliferation of antigen-specific T-cells in lymph nodes, while simultaneously reducing apoptosis in regulatory T cells (anti-inflammatory, suppressive T cells) – further mediated by a lower regulation of the gene Bcl-2.

Background References

1. Lei Q et al. Resistance Mechanisms of Anti-PD1/PDL1 Therapy in Solid Tumors. Front Cell Dev Biol. 2020 Jul

2. Tran-Nguyen VK et al. Structure-based virtual screening for PDL1 dimerizers: Evaluating generic scoring functions. Curr Res Struct Biol. 2022 Jun

Subcellular Location

Cell membrane, Early endosome membrane, Recycling endosome membrane.

UNIPROT

SwissProt: Q9EP73 Mouse

Synonyms

B7 H antibody

B7 H1 antibody

B7 homolog 1 antibody

B7-H1 antibody

B7H antibody

B7H1 antibody

CD 274 antibody

CD-274 antibody

CD274 antibody

CD274 antigen antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of PD-L1 on different lysates with Rabbit anti-PD-L1 antibody (<a href="/products/HA750974" style="font-weight: bold;text-decoration: underline;">HA750974</a>) at 1/2,000 dilution.<br /><br />Lane 1: RAW264.7 cell lysate<br />Lane 2: RAW264.7 treated with 10μg/mL LPS for 8 hours cell lysate<br />Lane 3: J774A.1 cell lysate<br />Lane 4: J774A.1 treated with 1μg/mL LPS for 24 hours cell lysate<br />Lane 5: Mouse spleen tissue lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 33 kDa<br />Observed band size: 45-60 kDa<br /><br />Exposure time: 46 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750974" style="font-weight: bold;text-decoration: underline;">HA750974</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of PD-L1 on different lysates with Rabbit anti-PD-L1 antibody (HA750974) at 1/2,000 dilution.

    Lane 1: RAW264.7 cell lysate
    Lane 2: RAW264.7 treated with 10μg/mL LPS for 8 hours cell lysate
    Lane 3: J774A.1 cell lysate
    Lane 4: J774A.1 treated with 1μg/mL LPS for 24 hours cell lysate
    Lane 5: Mouse spleen tissue lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 33 kDa
    Observed band size: 45-60 kDa

    Exposure time: 46 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750974) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse thymus tissue with Rabbit anti-PD-L1 antibody (<a href="/products/HA750974" style="font-weight: bold;text-decoration: underline;">HA750974</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750974" style="font-weight: bold;text-decoration: underline;">HA750974</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue with Rabbit anti-PD-L1 antibody (HA750974) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750974) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-PD-L1 antibody (<a href="/products/HA750974" style="font-weight: bold;text-decoration: underline;">HA750974</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750974" style="font-weight: bold;text-decoration: underline;">HA750974</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-PD-L1 antibody (HA750974) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750974) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"