APP/beta Amyloid Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH04-90] (HA750977)
Catalog# HA750977
Rabbit Monoclonal to APP/β-Amyloid
Tested for
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WB
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IF-Cell
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IHC-P
Reacts with
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Human
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Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of APP/β-Amyloid on different lysates with Rabbit anti-APP/β-Amyloid antibody (HA750977) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 100ng/mL Nocodazole for 17 hours cell lysate
Lane 3: SH-SY5Y cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 87 kDa
Observed band size: 87-120 kDa
Exposure time: 1 minute 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750977) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of APP/β-Amyloid on different lysates with Rabbit anti-APP/β-Amyloid antibody (HA750977) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 100ng/mL Nocodazole for 17 hours cell lysate
Lane 3: SH-SY5Y cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 87 kDa
Observed band size: 87-120 kDa
Exposure time: 1 minute 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750977) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.