NSUN5 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH05-07] (HA750980)
Catalog# HA750980
Rabbit Monoclonal to NSUN5
Tested for
-
WB
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of NSUN5 on different lysates with Rabbit anti-NSUN5 antibody (HA750980) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: Caco-2 cell lysate
Lane 3: K-562 cell lysate
Lane 4: A431 cell lysate
Lane 5: MEF cell lysate
Lane 6: PC-12 cell lysate
Lane 7: Human kidney tissue lysate
Lane 8: Mouse kidney tissue lysate
Lane 9: Mouse spleen tissue lysate
Lane 10: Rat spleen tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750980) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: Caco-2 cell lysate
Lane 3: K-562 cell lysate
Lane 4: A431 cell lysate
Lane 5: MEF cell lysate
Lane 6: PC-12 cell lysate
Lane 7: Human kidney tissue lysate
Lane 8: Mouse kidney tissue lysate
Lane 9: Mouse spleen tissue lysate
Lane 10: Rat spleen tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750980) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.