RAC1 Recombinant Rabbit Monoclonal Antibody [PSH05-09] - BSA and Azide free
Catalog# HA750982
RAC1 Recombinant Rabbit Monoclonal Antibody [PSH05-09] - BSA and Azide free
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WB
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IF-Cell
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Human
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Rat
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unconjugated
Overview
Product Name
RAC1 Recombinant Rabbit Monoclonal Antibody [PSH05-09] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human RAC1 aa 1-192 / 192.
Species Reactivity
Human, Rat
Validated Applications
WB, IF-Cell
Target Molecular Weight
Predicted band size: 21 kDa
Positive Control
HEK-293 cell lysate, Huh7 cell lysate, HUVEC cell lysate, HL-60 cell lysate, HeLa cell lysate, PC-12 cell lysate, HL-60.
Conjugation
unconjugated
Clone Number
PSH05-09
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Rac1, also known as Ras-related C3 botulinum toxin substrate 1, is a protein found in human cells. It is encoded by the RAC1 gene. This gene can produce a variety of alternatively spliced versions of the Rac1 protein, which appear to carry out different functions. Rac1 is a small (~21 kDa) signalling G protein (more specifically a GTPase), and is a member of the Rac subfamily of the family Rho family of GTPases. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of GLUT4 translocation to glucose uptake, cell growth, cytoskeletal reorganization, antimicrobial cytotoxicity, and the activation of protein kinases. Rac1 is a pleiotropic regulator of many cellular processes, including the cell cycle, cell-cell adhesion, motility (through the actin network), and of epithelial differentiation (proposed to be necessary for maintaining epidermal stem cells).
Background References
1. Liu J et al. Metabolic enzyme LDHA activates Rac1 GTPase as a noncanonical mechanism to promote cancer. Nat Metab. 2022 Dec
2. Liang J et al. Rac1, A Potential Target for Tumor Therapy. Front Oncol. 2021 May
Subcellular Location
Cell membrane, Cell projection, Cytoplasm, Membrane, Nucleus, Synapse.
Synonyms
Cell migration inducing gene 5 protein antibody
Cell migration-inducing gene 5 protein antibody
MGC111543 antibody
MIG5 antibody
Migration inducing gene 5 antibody
Migration inducing protein 5 antibody
p21 Rac1 antibody
p21-Rac1 antibody
Rac 1 antibody
RAC1 antibody
ExpandImages
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This data was developed using HA722225, the same antibody clone in a different buffer formulation.
Western blot analysis of RAC1 on different lysates with Rabbit anti-RAC1 antibody (HA722225) at 1/2,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: Huh7 cell lysate
Lane 3: HUVEC cell lysate
Lane 4: HL-60 cell lysate
Lane 5: HeLa cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722225) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA722225, the same antibody clone in a different buffer formulation.
Western blot analysis of RAC1 on different lysates with Rabbit anti-RAC1 antibody (HA722225) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722225) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA722225, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of HL-60 cells labeling RAC1 with Rabbit anti-RAC1 antibody (HA722225) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RAC1 antibody (HA722225) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
☑ Knockdown (KD)
This data was developed using HA722225, the same antibody clone in a different buffer formulation.
Western blot analysis of RAC1 on different lysates with Rabbit anti-RAC1 antibody (HA722225) at 1/1,000 dilution.
Lane 1: Huh7-parental cell lysate
Lane 2: Huh7-RAC1 KD cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 1 minute 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722225) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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