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Presenilin 2 / AD5 Recombinant Rabbit Monoclonal Antibody [PSH05-18] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750989

Presenilin 2 / AD5 Recombinant Rabbit Monoclonal Antibody [PSH05-18] - BSA and Azide free

Application

  • WB

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Presenilin 2 / AD5 Recombinant Rabbit Monoclonal Antibody [PSH05-18] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Presenilin 2 aa 300-400.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 50 kDa

Positive Control

HeLa cell lysate, HepG2 cell lysate, A549 cell lysate, 293T cell lysate, SH-SY5Y cell lysate, RAW264.7 cell lysate, Neuro-2a cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat liver tissue lysate.

Conjugation

unconjugated

Clone Number

PSH05-18

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IP

  • 1-2μg/sample

Target

Function

Presenilin-2 is a protein that (in humans) is encoded by the PSEN2 gene. Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1; PSEN2) or the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that they either directly regulate gamma-secretase activity or themselves are protease enzymes. Two alternative transcripts of PSEN2 have been identified. In melanocytic cells PSEN2 gene expression may be regulated by MITF.

Background References

1. Barazzuol L et al. Unraveling Presenilin 2 Functions in a Knockout Zebrafish Line to Shed Light into Alzheimer\'s Disease Pathogenesis. Cells. 2023 Jan

2. Nam H et al. Presenilin 2 N141I mutation induces hyperactive immune response through the epigenetic repression of REV-ERBalpha. Nat Commun. 2022 Apr

Subcellular Location

Endoplasmic reticulum membrane, Golgi apparatus membrane.

UNIPROT

SwissProt: P49810 Human

SwissProt: Q61144 Mouse

SwissProt: O88777 Rat

Synonyms

AD3L antibody

AD3LP antibody

AD4 antibody

AD5 antibody

Alzheimer disease 4 antibody

CMD1V antibody

E5-1 antibody

OTTHUMP00000035671 antibody

OTTHUMP00000035672 antibody

OTTHUMP00000228286 antibody

Expand

Images

  • Western blot analysis of Presenilin 2 / AD5 on different lysates with Rabbit anti-Presenilin 2 / AD5 antibody (<a href="/products/HA750989" style="font-weight: bold;text-decoration: underline;">HA750989</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: HepG2 cell lysate (20 µg/Lane)<br />Lane 3: A549 cell lysate (20 µg/Lane)<br />Lane 4: 293T cell lysate (20 µg/Lane)<br />Lane 5: SH-SY5Y cell lysate (20 µg/Lane)<br />Lane 6: RAW264.7 cell lysate (20 µg/Lane)<br />Lane 7: Neuro-2a cell lysate (20 µg/Lane)<br />Lane 8: C2C12 cell lysate (20 µg/Lane)<br />Lane 9: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 10: PC-12 cell lysate (20 µg/Lane)<br />Lane 11: C6 cell lysate (20 µg/Lane)<br />Lane 12: Human brain tissue lysate (40 µg/Lane)<br />Lane 13: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 14: Rat liver tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 50 kDa<br />Observed band size: 23 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750989" style="font-weight: bold;text-decoration: underline;">HA750989</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Presenilin 2 / AD5 on different lysates with Rabbit anti-Presenilin 2 / AD5 antibody (HA750989) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: HepG2 cell lysate (20 µg/Lane)
    Lane 3: A549 cell lysate (20 µg/Lane)
    Lane 4: 293T cell lysate (20 µg/Lane)
    Lane 5: SH-SY5Y cell lysate (20 µg/Lane)
    Lane 6: RAW264.7 cell lysate (20 µg/Lane)
    Lane 7: Neuro-2a cell lysate (20 µg/Lane)
    Lane 8: C2C12 cell lysate (20 µg/Lane)
    Lane 9: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 10: PC-12 cell lysate (20 µg/Lane)
    Lane 11: C6 cell lysate (20 µg/Lane)
    Lane 12: Human brain tissue lysate (40 µg/Lane)
    Lane 13: Mouse brain tissue lysate (40 µg/Lane)
    Lane 14: Rat liver tissue lysate (40 µg/Lane)

    Predicted band size: 50 kDa
    Observed band size: 23 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750989) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Presenilin 2 / AD5 was immunoprecipitated from 0.2 mg HeLa cell lysate with <a href="/products/HA750989" style="font-weight: bold;text-decoration: underline;">HA750989</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA750989" style="font-weight: bold;text-decoration: underline;">HA750989</a> at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: HeLa cell lysate (input)<br />Lane 2: <a href="/products/HA750989" style="font-weight: bold;text-decoration: underline;">HA750989</a> IP in HeLa cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA750989" style="font-weight: bold;text-decoration: underline;">HA750989</a> in HeLa cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 59 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    Presenilin 2 / AD5 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA750989 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750989 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HeLa cell lysate (input)
    Lane 2: HA750989 IP in HeLa cell lysate
    Lane 3: Rabbit IgG instead of HA750989 in HeLa cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 59 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"