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ATG4C Recombinant Rabbit Monoclonal Antibody [PSH05-23] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750994

ATG4C Recombinant Rabbit Monoclonal Antibody [PSH05-23] - BSA and Azide free

Application

  • WB

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

ATG4C Recombinant Rabbit Monoclonal Antibody [PSH05-23] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human ATG4C aa 1-458 / 458.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 52 kDa

Positive Control

293T cell lysate, Jurkat cell lysate, Raji cell lysate, A431 cell lysate, HeLa cell lysate, K-562 cell lysate, HL-60 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, COS-1 cell lysate.

Conjugation

unconjugated

Clone Number

PSH05-23

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IP

  • 1-2μg/sample

Target

Function

Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins. The protease activity is required for proteolytic activation of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8 proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy (By similarity). In addition to the protease activity, also mediates delipidation of ATG8 family proteins. Catalyzes delipidation of PE-conjugated forms of ATG8 proteins during macroautophagy. Compared to ATG4B, the major protein for proteolytic activation of ATG8 proteins, shows weaker ability to cleave the C-terminal amino acid of ATG8 proteins, while it displays stronger delipidation activity. In contrast to other members of the family, weakly or not involved in phagophore growth during mitophagy.

Background References

1. Tamargo-Gómez I et al. Analysis of ATG4C function in vivo. Autophagy. 2023 Nov

2. Wen ZP et al. Knockdown ATG4C inhibits gliomas progression and promotes temozolomide chemosensitivity by suppressing autophagic flux. J Exp Clin Cancer Res. 2019 Jul

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: Q96DT6 Human

SwissProt: Q811C2 Mouse

Entrez Gene: 313391 Rat

Synonyms

APG4 autophagy 4 homolog C (S. cerevisiae) antibody

APG4 autophagy 4 homolog C antibody

APG4 C antibody

APG4-C antibody

APG4C antibody

ATG 4C antibody

ATG4 autophagy related 4 homolog C (S. cerevisiae) antibody

ATG4 autophagy related 4 homolog C antibody

Atg4c antibody

ATG4C_HUMAN antibody

Expand

Images

  • Western blot analysis of ATG4C on different lysates with Rabbit anti-ATG4C antibody (<a href="/products/HA750994" style="font-weight: bold;text-decoration: underline;">HA750994</a>) at 1/2,000 dilution.<br /><br />Lane 1: 293T cell lysate (20 µg/Lane)<br />Lane 2: Jurkat cell lysate (20 µg/Lane)<br />Lane 3: Raji cell lysate (20 µg/Lane)<br />Lane 4: A431 cell lysate (20 µg/Lane)<br />Lane 5: HeLa cell lysate (20 µg/Lane)<br />Lane 6: K-562 cell lysate (20 µg/Lane)<br />Lane 7: HL-60 cell lysate (20 µg/Lane)<br />Lane 8: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 9: Rat brain tissue lysate (40 µg/Lane)<br />Lane 10: COS-1 cell lysate (20 µg/Lane)<br /><br />Predicted band size: 52 kDa<br />Observed band size: 52 kDa<br /><br />Exposure time: 30 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750994" style="font-weight: bold;text-decoration: underline;">HA750994</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ATG4C on different lysates with Rabbit anti-ATG4C antibody (HA750994) at 1/2,000 dilution.

    Lane 1: 293T cell lysate (20 µg/Lane)
    Lane 2: Jurkat cell lysate (20 µg/Lane)
    Lane 3: Raji cell lysate (20 µg/Lane)
    Lane 4: A431 cell lysate (20 µg/Lane)
    Lane 5: HeLa cell lysate (20 µg/Lane)
    Lane 6: K-562 cell lysate (20 µg/Lane)
    Lane 7: HL-60 cell lysate (20 µg/Lane)
    Lane 8: Mouse brain tissue lysate (40 µg/Lane)
    Lane 9: Rat brain tissue lysate (40 µg/Lane)
    Lane 10: COS-1 cell lysate (20 µg/Lane)

    Predicted band size: 52 kDa
    Observed band size: 52 kDa

    Exposure time: 30 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750994) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • ATG4C was immunoprecipitated from 0.2 mg Raji cell lysate with <a href="/products/HA750994" style="font-weight: bold;text-decoration: underline;">HA750994</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA750994" style="font-weight: bold;text-decoration: underline;">HA750994</a> at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: Raji cell lysate (input)<br />Lane 2: <a href="/products/HA750994" style="font-weight: bold;text-decoration: underline;">HA750994</a> IP in Raji cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA750994" style="font-weight: bold;text-decoration: underline;">HA750994</a> in Raji cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 21 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    ATG4C was immunoprecipitated from 0.2 mg Raji cell lysate with HA750994 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750994 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: Raji cell lysate (input)
    Lane 2: HA750994 IP in Raji cell lysate
    Lane 3: Rabbit IgG instead of HA750994 in Raji cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 21 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"