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Phospho-Tau (T181) Recombinant Rabbit Monoclonal Antibody [PSH05-42] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751001

Phospho-Tau (T181) Recombinant Rabbit Monoclonal Antibody [PSH05-42] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

With BSA and Azide
Predicted reactivity

  • Cynomolgus monkey

Predicted species support after-sales service
Conjugation

  • unconjugated

Overview

Product Name

Phospho-Tau (T181) Recombinant Rabbit Monoclonal Antibody [PSH05-42] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding T181 of human Tau protein(P10636-8).

Species Reactivity

Human, Mouse (Predicted: Cynomolgus monkey)

Predicted species support after-sales service

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 79 kDa

Positive Control

Human brain tissue lysate, human brain tissue, mouse brain tissue.

Conjugation

unconjugated

Clone Number

PSH05-42

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:50

Target

Function

The tau proteins (abbreviated from tubulin associated unit are a group of six highly soluble protein isoforms produced by alternative splicing from the gene MAPT (microtubule-associated protein tau). They have roles primarily in maintaining the stability of microtubules in axons and are abundant in the neurons of the central nervous system (CNS), where the cerebral cortex has the highest abundance. They are less common elsewhere but are also expressed at very low levels in CNS astrocytes and oligodendrocytes. Pathologies and dementias of the nervous system such as Alzheimer's disease and Parkinson's disease are associated with tau proteins that have become hyperphosphorylated insoluble aggregates called neurofibrillary tangles. The tau proteins were identified in 1975 as heat-stable proteins essential for microtubule assembly, and since then they have been characterized as intrinsically disordered proteins.

Background References

1. Karikari TK et al. Blood phospho-tau in Alzheimer disease: analysis, interpretation, and clinical utility. Nat Rev Neurol. 2022 Jul

2. Palmqvist S et al. Prediction of future Alzheimer\'s disease dementia using plasma phospho-tau combined with other accessible measures. Nat Med. 2021 Jun

Subcellular Location

Cytoplasm, cytosol, Cell membrane, cytoskeleton, Cell projection, axon, dendrite, Secreted.

UNIPROT

SwissProt: P10636-8 Human

SwissProt: P10637 Mouse

Synonyms

AI413597 antibody

AW045860 antibody

DDPAC antibody

FLJ31424 antibody

FTDP 17 antibody

G protein beta1/gamma2 subunit interacting factor 1 antibody

MAPT antibody

MAPTL antibody

MGC134287 antibody

MGC138549 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-Tau (T181) on different lysates with Rabbit anti-Phospho-Tau (T181) antibody (<a href="/products/HA751001" style="font-weight: bold;text-decoration: underline;">HA751001</a>) at 1/1,000 dilution.<br /><br />Lane 1: Human brain tissue lysate<br />Lane 2: Human brain tissue lysate, the membrane treated with λpp for 1 hour<br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 79 kDa<br />Observed band size: 70 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751001" style="font-weight: bold;text-decoration: underline;">HA751001</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-Tau (T181) on different lysates with Rabbit anti-Phospho-Tau (T181) antibody (HA751001) at 1/1,000 dilution.

    Lane 1: Human brain tissue lysate
    Lane 2: Human brain tissue lysate, the membrane treated with λpp for 1 hour
    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 79 kDa
    Observed band size: 70 kDa

    Exposure time: 3 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751001) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Phospho-Tau (T181) antibody (<a href="/products/HA751001" style="font-weight: bold;text-decoration: underline;">HA751001</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751001" style="font-weight: bold;text-decoration: underline;">HA751001</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Phospho-Tau (T181) antibody (HA751001) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751001) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-Tau (T181) antibody (<a href="/products/HA751001" style="font-weight: bold;text-decoration: underline;">HA751001</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751001" style="font-weight: bold;text-decoration: underline;">HA751001</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-Tau (T181) antibody (HA751001) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751001) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"