NMDAR2B Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH05-46] (HA751005)
Catalog# HA751005
Rabbit Monoclonal to NMDAR2B
Tested for
-
WB
-
IHC-P
-
IHC-Fr
Reacts with
-
Human
-
Mouse
-
Rat
-
Cynomolgus monkey
-
Pig
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of NMDAR2B on different lysates with Rabbit anti-NMDAR2B antibody (HA751005) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate (no heat)
Lane 2: Mouse hippocampus tissue lysate
Lane 3: Mouse liver tissue lysate (negative)
Lane 4: Rat brain tissue lysate (no heat)
Lane 5: Rat hippocampus tissue lysate
Lane 6: Rat liver tissue lysate (negative)
Lane 7: Human brain tissue lysate
Lane 8: Human liver tissue lysate (negative)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 166 kDa
Observed band size: 115/180 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751005) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of NMDAR2B on different lysates with Rabbit anti-NMDAR2B antibody (HA751005) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate (no heat)
Lane 2: Mouse hippocampus tissue lysate
Lane 3: Mouse liver tissue lysate (negative)
Lane 4: Rat brain tissue lysate (no heat)
Lane 5: Rat hippocampus tissue lysate
Lane 6: Rat liver tissue lysate (negative)
Lane 7: Human brain tissue lysate
Lane 8: Human liver tissue lysate (negative)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 166 kDa
Observed band size: 115/180 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751005) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.