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Niemann Pick C2 Recombinant Rabbit Monoclonal Antibody [PSH05-52] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751011

Niemann Pick C2 Recombinant Rabbit Monoclonal Antibody [PSH05-52] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Niemann Pick C2 Recombinant Rabbit Monoclonal Antibody [PSH05-52] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Niemann Pick C2 aa 1-151 / 151.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 17 kDa

Positive Control

HepG2 cell lysate, HEK-293 cell lysate, RAW264.7 cell lysate, C2C12 cell lysate, C6 cell lysate, mouse kidney tissue lysate, mouse spleen tissue lysate, rat kidney tissue lysate, rat spleen tissue lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue.

Conjugation

unconjugated

Clone Number

PSH05-52

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

The epididymal secretory protein E1, also known as NPC2 (Niemann-Pick intracellular cholesterol transporter 2), is one of two main lysosomal transport proteins that assist in the regulation of cellular cholesterol by exportation of LDL-derived cholesterol from lysosomes. Lysosomes have digestive enzymes that allow it to break down LDL particles to LDL-derived cholesterol once the LDL particle is engulfed into the cell via receptor mediated endocytosis. NPC2 works cooperatively with the NPC1 protein to facilitate the exportation of LDL-derived cholesterol out of the lysosome to regulate the concentrations of lipids and cholesterol in the body. Epididymal secretory protein E1 is a protein associated with Niemann-Pick disease, type C, which is one of the 3 types of the Niemann-Pick diseases (Type A, B, and C). This disease can lead to an over accumulation of cholesterol and lipids in different types of tissues, including the brain. It is caused by a mutation in the NPC2 gene that impairs the body's ability to transport lipids or cholesterol intracellularly.

Background References

1. Tseng WC et al. The role of Niemann-Pick type C2 in zebrafish embryonic development. Development. 2021 Apr

2. Juhl AD et al. Niemann Pick C2 protein enables cholesterol transfer from endo-lysosomes to the plasma membrane for efflux by shedding of extracellular vesicles. Chem Phys Lipids. 2021 Mar

Subcellular Location

Secreted, Endoplasmic reticulum, Lysosome.

UNIPROT

SwissProt: P61916 Human

SwissProt: Q9Z0J0 Mouse

Entrez Gene: 286898 Rat

Synonyms

EDDM1 antibody

Epididymal protein 1 antibody

Epididymal secretory protein antibody

Epididymal secretory protein E1 antibody

hE1 antibody

Human epididymis-specific protein 1 antibody

Niemann-Pick disease type C2 antibody

Niemann-Pick disease type C2 protein antibody

NPC intracellular cholesterol transporter 2 antibody

NPC2 antibody

Expand

Images

  • This data was developed using <a href="/products/HA722294" style="font-weight: bold;text-decoration: underline;">HA722294</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of Niemann Pick C2 on different lysates with Rabbit anti-Niemann Pick C2 antibody (<a href="/products/HA722294" style="font-weight: bold;text-decoration: underline;">HA722294</a>) at 1/5,000 dilution.<br /><br />Lane 1: HepG2 cell lysate (10 µg/Lane)<br />Lane 2: HEK-293 cell lysate (10 µg/Lane)<br />Lane 3: RAW264.7 cell lysate (10 µg/Lane)<br />Lane 4: C2C12 cell lysate (10 µg/Lane)<br />Lane 5: C6 cell lysate (10 µg/Lane)<br />Lane 6: Mouse kidney tissue lysate (20 µg/Lane)<br />Lane 7: Mouse spleen tissue lysate (20 µg/Lane)<br />Lane 8: Rat kidney tissue lysate (20 µg/Lane)<br />Lane 9: Rat spleen tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 16 kDa<br />Observed band size: 16-18 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722294" style="font-weight: bold;text-decoration: underline;">HA722294</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    This data was developed using HA722294, the same antibody clone in a different buffer formulation.
    Western blot analysis of Niemann Pick C2 on different lysates with Rabbit anti-Niemann Pick C2 antibody (HA722294) at 1/5,000 dilution.

    Lane 1: HepG2 cell lysate (10 µg/Lane)
    Lane 2: HEK-293 cell lysate (10 µg/Lane)
    Lane 3: RAW264.7 cell lysate (10 µg/Lane)
    Lane 4: C2C12 cell lysate (10 µg/Lane)
    Lane 5: C6 cell lysate (10 µg/Lane)
    Lane 6: Mouse kidney tissue lysate (20 µg/Lane)
    Lane 7: Mouse spleen tissue lysate (20 µg/Lane)
    Lane 8: Rat kidney tissue lysate (20 µg/Lane)
    Lane 9: Rat spleen tissue lysate (20 µg/Lane)

    Predicted band size: 16 kDa
    Observed band size: 16-18 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722294) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • This data was developed using <a href="/products/HA722294" style="font-weight: bold;text-decoration: underline;">HA722294</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Niemann Pick C2 antibody (<a href="/products/HA722294" style="font-weight: bold;text-decoration: underline;">HA722294</a>) at 1/100,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722294" style="font-weight: bold;text-decoration: underline;">HA722294</a>) at 1/100,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA722294, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Niemann Pick C2 antibody (HA722294) at 1/100,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722294) at 1/100,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA722294" style="font-weight: bold;text-decoration: underline;">HA722294</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Niemann Pick C2 antibody (<a href="/products/HA722294" style="font-weight: bold;text-decoration: underline;">HA722294</a>) at 1/100,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722294" style="font-weight: bold;text-decoration: underline;">HA722294</a>) at 1/100,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA722294, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Niemann Pick C2 antibody (HA722294) at 1/100,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722294) at 1/100,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA722294" style="font-weight: bold;text-decoration: underline;">HA722294</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Niemann Pick C2 antibody (<a href="/products/HA722294" style="font-weight: bold;text-decoration: underline;">HA722294</a>) at 1/300,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722294" style="font-weight: bold;text-decoration: underline;">HA722294</a>) at 1/300,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA722294, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Niemann Pick C2 antibody (HA722294) at 1/300,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722294) at 1/300,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"