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IMP3 Recombinant Rabbit Monoclonal Antibody [PSH05-54] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751013

IMP3 Recombinant Rabbit Monoclonal Antibody [PSH05-54] - BSA and Azide free

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

IMP3 Recombinant Rabbit Monoclonal Antibody [PSH05-54] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human IMP3 aa 530-579 / 579.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 64 kDa

Positive Control

293T cell lysate, HeLa cell lysate, Jurkat cell lysate, K-562 cell lysate, HT-29 cell lysate, NIH/3T3 cell lysate, human lung cancer tissue, human placenta tissue.

Conjugation

unconjugated

Clone Number

PSH05-54

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

  • IF-Tissue

  • 1:200

Target

Function

Insulin-like growth factor 2 mRNA-binding protein 3 is a protein that in humans is encoded by the IGF2BP3 gene. The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes.

Background References

1. Wan W et al. METTL3/IGF2BP3 axis inhibits tumor immune surveillance by upregulating N6-methyladenosine modification of PD-L1 mRNA in breast cancer. Mol Cancer. 2022 Feb

2. Pan Z et al. EWSR1-induced circNEIL3 promotes glioma progression and exosome-mediated macrophage immunosuppressive polarization via stabilizing IGF2BP3. Mol Cancer. 2022 Jan

Subcellular Location

Nucleus, Cytoplasm, P-body, Stress granule.

UNIPROT

SwissProt: O00425 Human

SwissProt: Q9CPN8 Mouse

Synonyms

Cancer/testis antigen 98 antibody

CT98 antibody

DKFZp686F1078 antibody

hKOC antibody

IF2B3_HUMAN antibody

IGF II mRNA binding protein 3 antibody

IGF-II mRNA-binding protein 3 antibody

IGF2 mRNA binding protein 3 antibody

IGF2 mRNA-binding protein 3 antibody

IGF2BP3 antibody

Expand

Images

  • Western blot analysis of IMP3 on different lysates with Rabbit anti-IMP3 antibody (<a href="/products/HA751013" style="font-weight: bold;text-decoration: underline;">HA751013</a>) at 1/1,000 dilution.<br /><br />Lane 1: 293T cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: Jurkat cell lysate<br />Lane 4: K-562 cell lysate<br />Lane 5: HT-29 cell lysate<br />Lane 6: NIH/3T3 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 64 kDa<br />Observed band size: 70 kDa<br /><br />Exposure time: 3 minutes; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751013" style="font-weight: bold;text-decoration: underline;">HA751013</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of IMP3 on different lysates with Rabbit anti-IMP3 antibody (HA751013) at 1/1,000 dilution.

    Lane 1: 293T cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: K-562 cell lysate
    Lane 5: HT-29 cell lysate
    Lane 6: NIH/3T3 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 64 kDa
    Observed band size: 70 kDa

    Exposure time: 3 minutes; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751013) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of IMP3 on different lysates with Rabbit anti-IMP3 antibody (<a href="/products/HA751013" style="font-weight: bold;text-decoration: underline;">HA751013</a>) at 1/1,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-IMP3 KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 64 kDa<br />Observed band size: 64 kDa<br /><br />Exposure time: 2 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751013" style="font-weight: bold;text-decoration: underline;">HA751013</a>) at 1/1,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of IMP3 on different lysates with Rabbit anti-IMP3 antibody (HA751013) at 1/1,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-IMP3 KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 64 kDa
    Observed band size: 64 kDa

    Exposure time: 2 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751013) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-IMP3 antibody (<a href="/products/HA751013" style="font-weight: bold;text-decoration: underline;">HA751013</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751013" style="font-weight: bold;text-decoration: underline;">HA751013</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-IMP3 antibody (HA751013) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751013) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-IMP3 antibody (<a href="/products/HA751013" style="font-weight: bold;text-decoration: underline;">HA751013</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751013" style="font-weight: bold;text-decoration: underline;">HA751013</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-IMP3 antibody (HA751013) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751013) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"