MFGE8 Recombinant Rabbit Monoclonal Antibody [PSH05-75] - BSA and Azide free
Catalog# HA751025
MFGE8 Recombinant Rabbit Monoclonal Antibody [PSH05-75] - BSA and Azide free
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WB
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Human
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unconjugated
Overview
Product Name
MFGE8 Recombinant Rabbit Monoclonal Antibody [PSH05-75] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human MFGE8 aa 1-387.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 43 kDa
Positive Control
A549 cell lysate, A431 cell lysate, HeLa cell lysate, MCF7 cell lysate, SK-Br-3 cell lysate, BT-20 cell lysate.
Conjugation
unconjugated
Clone Number
PSH05-75
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Milk fat globule-EGF factor 8 protein (Mfge8), also known as lactadherin, is a protein that in humans is encoded by the MFGE8 gene. Mfge8 is a secreted protein found in vertebrates, including mammals as well as birds. MFGE8 may function as a cell adhesion protein to connect smooth muscle to elastic fibers in arteries. An amyloid fragment of MFGE8 known as medin accumulates in the aorta with aging. MFGE8 in the vasculature of adults can induce recovery from ischemia by facilitating angiogenesis. It has been suggested that antagonizing MFGE8-induced angiogenesis could be a way of fighting cancer. MFGE8 contains a phosphatidylserine (PS) binding domain, as well as an arginine-glycine-aspartic acid motif, which enables the binding to integrins. MFGE8 binds PS, which is exposed on the surface of apoptotic cells. Opsonization of the apoptotic cells and binding to integrins on the surface of phagocytic cells, mediates the engulfment of the dead cell.
Background References
1. Liu B et al. Targeting MFGE8 secreted by cancer-associated fibroblasts blocks angiogenesis and metastasis in esophageal squamous cell carcinoma. Proc Natl Acad Sci U S A. 2023 Oct
2. Datta R et al. MFGE8 inhibits insulin signaling through PTP1B. bioRxiv [Preprint]. 2023 Jun
Subcellular Location
Membrane, Secreted, Cytoplasmic vesicle, secretory vesicle, acrosome membrane.
Synonyms
BA46 antibody
Breast epithelial antigen BA46 antibody
EDIL1 antibody
HMFG antibody
hP47 antibody
HsT19888 antibody
Lactadherin antibody
Medin antibody
MFG-E8 antibody
MFGE8 antibody
ExpandImages
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This data was developed using HA722442, the same antibody clone in a different buffer formulation.
Western blot analysis of MFGE8 on different lysates with Rabbit anti-MFGE8 antibody (HA722442) at 1/1,000 dilution.
Lane 1: A549 cell lysate
Lane 2: A431 cell lysate
Lane 3: HeLa cell lysate
Lane 4: MCF7 cell lysate
Lane 5: SK-Br-3 cell lysate
Lane 6: BT-20 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 42 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722442) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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