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Pyrin Recombinant Rabbit Monoclonal Antibody [PSH06-12] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751050

Pyrin Recombinant Rabbit Monoclonal Antibody [PSH06-12] - BSA and Azide free

Application

  • WB

  • FC

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Pyrin Recombinant Rabbit Monoclonal Antibody [PSH06-12] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Pyrin aa 450-781.

Species Reactivity

Human

Validated Applications

WB, FC

Molecular Weight

Predicted band size: 86 kDa

Positive Control

THP-1 cell lysate, THP-1 treated with 1μg/mL LPS for 24 hours cell lysate, THP-1.

Conjugation

unconjugated

Clone Number

PSH06-12

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • FC

  • 1:1,000

Target

Function

Involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma. Organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1, ATG16L1, and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy. Acts as an autophagy receptor for the degradation of several inflammasome components, including CASP1, NLRP1 and NLRP3, hence preventing excessive IL1B- and IL18-mediated inflammation. However, it can also have a positive effect in the inflammatory pathway, acting as an innate immune sensor that triggers PYCARD/ASC specks formation, caspase-1 activation, and IL1B and IL18 production. Together with AIM2, also acts as a mediator of pyroptosis, necroptosis and apoptosis (PANoptosis), an integral part of host defense against pathogens, in response to bacterial infection. It is required for PSTPIP1-induced PYCARD/ASC oligomerization and inflammasome formation. Recruits PSTPIP1 to inflammasomes, and is required for PSTPIP1 oligomerization.

Background References

1. Papin S., Cuenin S., Agostini L., Martinon F., Werner S., Beer H.D., Grutter C., Grutter M., Tschopp J. The SPRY domain of Pyrin, mutated in familial Mediterranean fever patients, interacts with inflammasome components and inhibits proIL-1beta processing. Cell Death Differ. 14:1457-1466 (2007)

2. Kimura T., Jain A., Choi S.W., Mandell M.A., Schroder K., Johansen T., Deretic V. TRIM-mediated precision autophagy targets cytoplasmic regulators of innate immunity. J. Cell Biol. 210:973-989 (2015)

Subcellular Location

Cell projection. Cytoplasm. Cytoskeleton. Microtubule. Nucleus.

UNIPROT

SwissProt: O15553 Human

Synonyms

FMF antibody

Marenostrin antibody

Mediterranean fever antibody

Mediterranean fever protein antibody

MEF antibody

Mefv antibody

MEFV_HUMAN antibody

Pyrin antibody

TRIM20 antibody

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Pyrin on different lysates with Rabbit anti-Pyrin antibody (<a href="/products/HA751050" style="font-weight: bold;text-decoration: underline;">HA751050</a>) at 1/1,000 dilution.<br /><br />Lane 1: THP-1 cell lysate<br />Lane 2: THP-1 treated with 1μg/mL LPS for 24 hours cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 86 kDa<br />Observed band size: 86 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751050" style="font-weight: bold;text-decoration: underline;">HA751050</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Pyrin on different lysates with Rabbit anti-Pyrin antibody (HA751050) at 1/1,000 dilution.

    Lane 1: THP-1 cell lysate
    Lane 2: THP-1 treated with 1μg/mL LPS for 24 hours cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 86 kDa
    Observed band size: 86 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751050) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of THP-1 cells labeling Pyrin.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA751050" style="font-weight: bold;text-decoration: underline;">HA751050</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of THP-1 cells labeling Pyrin.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA751050, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"