Phospho-PLC-gamma-1 (Y783) Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH06-29] (HA751066)
Catalog# HA751066
Rabbit Monoclonal to Phospho-PLC-gamma-1 (Y783)
Tested for
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WB
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Phospho-PLC-gamma-1 (Y783) on different lysates with Rabbit anti-Phospho-PLC-gamma-1 (Y783) antibody (HA751066) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.
Lane 1: A431 cell lysate
Lane 2: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: NIH/3T3 starved overnight then add 100ng/mL PDGF for 5 minutes cell lysate
Lane 5: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 149 kDa
Observed band size: 149 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751066) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-PLC-gamma-1 (Y783) on different lysates with Rabbit anti-Phospho-PLC-gamma-1 (Y783) antibody (HA751066) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.
Lane 1: A431 cell lysate
Lane 2: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: NIH/3T3 starved overnight then add 100ng/mL PDGF for 5 minutes cell lysate
Lane 5: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 149 kDa
Observed band size: 149 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751066) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.