CSPP1 Recombinant Rabbit Monoclonal Antibody [PSH06-31] - BSA and Azide free
Catalog# HA751068
CSPP1 Recombinant Rabbit Monoclonal Antibody [PSH06-31] - BSA and Azide free
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WB
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IF-Cell
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Human
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unconjugated
Overview
Product Name
CSPP1 Recombinant Rabbit Monoclonal Antibody [PSH06-31] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human CSPP1 aa 951-1,256.
Species Reactivity
Human
Validated Applications
WB, IF-Cell
Target Molecular Weight
Predicted band size: 146 kDa
Positive Control
HeLa cell lysate, Jurkat cell lysate, K-562 cell lysate, HEK-293 cell lysate, HeLa cells treated with 2mM thymidine for 16 hours then recovery for 8 hours.
Conjugation
unconjugated
Clone Number
PSH06-31
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:100
Target
Function
This gene encodes a centrosome and spindle pole associated protein. The encoded protein plays a role in cell-cycle progression and spindle organization, regulates cytokinesis, interacts with Nephrocystin 8 and is required for cilia formation. Mutations in this gene result in primary cilia abnormalities and classical Joubert syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Background References
1. Wang W et al. Identifies microtubule-binding protein CSPP1 as a novel cancer biomarker associated with ferroptosis and tumor microenvironment. Comput Struct Biotechnol J. 2022 Jun
2. Yang G et al. Circ-CSPP1 knockdown suppresses hepatocellular carcinoma progression through miR-493-5p releasing-mediated HMGB1 downregulation. Cell Signal. 2021 Oct
Subcellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, spindle, spindle pole.
UNIPROT
Synonyms
Centrosome and spindle pole-associated protein 1
CSPP1
CSPP
Images
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Western blot analysis of CSPP1 on different lysates with Rabbit anti-CSPP1 antibody (HA751068) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: K-562 cell lysate
Lane 4: HEK-293 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 146 kDa
Observed band size: 100-150 kDa
Exposure time: 1 minute 46 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751068) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Immunocytochemistry analysis of HeLa cells treated with 2mM thymidine for 16 hours then recovery for 8 hours labeling CSPP1 with Rabbit anti-CSPP1 antibody (HA751068) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CSPP1 antibody (HA751068) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"