MMP-9 Recombinant Rabbit Monoclonal Antibody [PSH06-33] - BSA and Azide free
Catalog# HA751070
MMP-9 Recombinant Rabbit Monoclonal Antibody [PSH06-33] - BSA and Azide free
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WB
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IHC-P
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Human
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unconjugated
Overview
Product Name
MMP-9 Recombinant Rabbit Monoclonal Antibody [PSH06-33] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human MMP-9 aa 1-707.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 78 kDa
Positive Control
U-2 OS treated with 200nM TPA for 24 hours cell lysate, human spleen tissue, human tonsil tissue.
Conjugation
unconjugated
Clone Number
PSH06-33
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, angiogenesis, bone development, wound healing, cell migration, learning and memory, as well as in pathological processes, such as arthritis, intracerebral hemorrhage, and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens and other extracellular matrix proteins. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Thrombospondins, intervertebral disc proteins, regulate interaction with matrix metalloproteinases (MMPs) 2 and 9, which are key effectors of ECM remodeling.
Background References
1. Cai N et al. Targeting MMP9 in CTNNB1 mutant hepatocellular carcinoma restores CD8(+) T cell-mediated antitumour immunity and improves anti-PD-1 efficacy. Gut. 2024 May
2. Augoff K et al. MMP9: A Tough Target for Targeted Therapy for Cancer. Cancers (Basel). 2022 Apr
Subcellular Location
Secreted, extracellular space, extracellular matrix.
UNIPROT
Synonyms
82 kDa matrix metalloproteinase-9 antibody
92 kDa gelatinase antibody
92 kDa type IV collagenase antibody
CLG 4B antibody
CLG4B antibody
Collagenase Type 4 beta antibody
Collagenase type IV 92 KD antibody
EC 3.4.24.35 antibody
Gelatinase 92 KD antibody
Gelatinase B antibody
ExpandImages
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☑ Cell treatment (CT)
This data was developed using HA722665, the same antibody clone in a different buffer formulation.
Western blot analysis of MMP-9 on different lysates with Rabbit anti-MMP-9 antibody (HA722665) at 1/2,000 dilution.
Lane 1: U-2 OS cell lysate
Lane 2: U-2 OS treated with 200nM TPA for 24 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 78 kDa
Observed band size: 78/92 kDa
Exposure time: 3 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722665) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA722665, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-MMP-9 antibody (HA722665) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722665) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA722665, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-MMP-9 antibody (HA722665) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722665) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"