CD133 Recombinant Rabbit Monoclonal Antibody [PSH06-48] - BSA and Azide free
Catalog# HA751072
CD133 Recombinant Rabbit Monoclonal Antibody [PSH06-48] - BSA and Azide free
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WB
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IHC-P
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IHC-Fr
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Mouse
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unconjugated
Overview
Product Name
CD133 Recombinant Rabbit Monoclonal Antibody [PSH06-48] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within
Species Reactivity
Mouse
Validated Applications
WB, IHC-P, IHC-Fr
Target Molecular Weight
Predicted band size: 97 kDa
Positive Control
Mouse kidney tissue lysate, mouse colon tissue.
Conjugation
unconjugated
Clone Number
PSH06-48
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
CD133 antigen, also known as prominin-1, is a glycoprotein that in humans is encoded by the PROM1 gene. It is a member of pentaspan transmembrane glycoproteins, which specifically localize to cellular protrusions. When embedded in the cell membrane, the membrane topology of prominin-1 is such that the N-terminus extends into the extracellular space and the C-terminus resides in the intracellular compartment. The protein consists of five transmembrane segments, with the first and second segments and the third and fourth segments connected by intracellular loops while the second and third as well as fourth and fifth transmembrane segments are connected by extracellular loops. CD133 is the most commonly used marker for isolation of cancer stem cell (CSC) population from different tumors, mainly from various gliomas and carcinomas. CD133+ melanoma cells are considered a subpopulation of CSC and play a critical role in recurrence. Moreover, CD133+ melanoma cells are immunogenic and can be used as an antimelanoma vaccination. In mice the vaccination with CD133+ melanoma cells mediated strong anti-tumor activity that resulted in the eradication of parental melanoma cells. In addition, it has also been shown that CD133+ melanoma cells preferentially express the RNA helicase DDX3X . As DDX3X also is an immunogenic protein, the same anti-melanoma vaccination strategy can be employed to give therapeutic antitumor immunity in mice.
Background References
1. Kim MY et al. Accumulation of low-dose BIX01294 promotes metastatic potential of U251 glioblastoma cells. Oncol Lett 13:1767-1774 (2017).
2. Xi G et al. Targeting CD133 improves chemotherapeutic efficacy of recurrent pediatric pilocytic astrocytoma following prolonged chemotherapy. Mol Cancer 16:21 (2017).
Subcellular Location
Endoplasmic reticulum. Plasma membrane. Cell projection.
UNIPROT
Synonyms
AC133 antibody
Antigen AC133 antibody
CD133 antibody
CORD12 antibody
Hematopoietic stem cell antigen antibody
hProminin antibody
MCDR2 antibody
MSTP061 antibody
OTTHUMP00000217744 antibody
OTTHUMP00000217745 antibody
ExpandImages
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☑ Cell treatment (CT)
This data was developed using HA722667, the same antibody clone in a different buffer formulation.
Western blot analysis of CD133 on different lysates with Rabbit anti-CD133 antibody (HA722667) at 1/1,000 dilution.
Lane 1: Mouse kidney tissue lysate
Lane 2: Mouse kidney tissue lysate treated with deglycosylation
Lysates/proteins at 20 µg/Lane.
Predicted band size: 97 kDa
Observed band size: 120/97 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722667) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA722667, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-CD133 antibody (HA722667) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722667) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA722667, the same antibody clone in a different buffer formulation.
Immunofluorescence analysis of frozen mouse colon tissue with Rabbit anti-CD133 antibody (HA722667) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722667, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"