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Phospho-EEF2 (T56) Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH06-36] (HA751075)

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Catalog# HA751075

Rabbit Monoclonal to Phospho-EEF2 (T56)

Tested for

  • WB

  • IHC-P

  • IF-Cell

Reacts with

  • Human

  • Mouse

  • Rat

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free

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Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Phospho-EEF2 (T56) Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH06-36] (HA751075)

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding thr56 of human eef2.

Host

Rabbit

Modification

Phospho

Modification Site

T56

Positive Control

HeLa starved for 24 hours then add 10μM forskolin for 1 hour cell lysate, NIH/3T3 cell lysate, NIH/3T3 starved for 24 hours then add 10μM forskolin for 1 hour cell lysate, PC-12 treated with 20μM forskolin for 1 hour then add 100nM Calyculin A for 30 minutes cell lysate, HepG2 cell lysate, rat liver tissue, HeLa cells starved for 24 hours then add 10μM forskolin for 1 hour, PC-12 cells treated with 20μM forskolin for 1 hour then add 100nM Calyculin A for 30 minutes.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

PSH06-36

APPLICATION DILUTION

  • WB

  • 1:2,000

  • IHC-P

  • 1:500

  • IF-Cell

  • 1:100

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 95 kDa

Isotype

IgG

TARGET

UNIPROT #

P13639

PROTEIN NAME

Phospho-EEF2 (T56)

SYNONYMS

EEF 2 antibody;Eef2 antibody;EF-2 antibody;EF2 antibody;EF2_HUMAN antibody;Elongation factor 2 antibody;Eukaryotic translation elongation factor 2 antibody;Polypeptidyl tRNA translocase antibody;SCA26 antibody

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

Eukaryotic elongation factor 2 is a protein that in humans is encoded by the EEF2 gene. It is the archaeal and eukaryotic counterpart of bacterial EF-G. This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the ribosome. This protein is completely inactivated by EF-2 kinase phosphorylation. aEF2/eEF2 found in most archaea and eukaryotes, including humans, contains a post translationally modified histidine diphthamide. It is the target of diphtheria toxin (from Corynebacterium diphtheriae), and exotoxin A (from Pseudomonas aeruginosa). The inactivation of EF-2 by toxins inhibits protein production in the host, causing symptoms due to loss of function in affected cells.

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