Cbx8 Recombinant Rabbit Monoclonal Antibody [PSH06-61] - BSA and Azide free

This data was developed using HA722734, the same antibody clone in a different buffer formulation.
Western blot analysis of Cbx8 on different lysates with Rabbit anti-Cbx8 antibody (HA722734) at 1/1,000 dilution.

Lane 1: HCT 116 cell lysate
Lane 2: HeLa cell lysate
Lane 3: SW620 cell lysate
Lane 4: U-2 OS cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: SP2/0 cell lysate
Lane 7: PC-12 cell lysate
Lane 8: C6 cell lysate
Lane 9: COS-1 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 43/40 kDa
Observed band size: 43/40 kDa

Exposure time: 20 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722734) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

This data was developed using HA722734, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of HeLa cells labeling Cbx8 with Rabbit anti-Cbx8 antibody (HA722734) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cbx8 antibody (HA722734) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

This data was developed using HA722734, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of NIH/3T3 cells labeling Cbx8 with Rabbit anti-Cbx8 antibody (HA722734) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cbx8 antibody (HA722734) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

This data was developed using HA722734, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of HeLa cells labeling Cbx8.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA722734, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).