HEXB Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH06-83] (HA751106)
Catalog# HA751106
Rabbit Monoclonal to HEXB
Tested for
-
WB
-
IHC-P
-
IF-Cell
-
FC
-
IF-Tissue
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of HEXB on different lysates with Rabbit anti-HEXB antibody (HA751106) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: HeLa cell lysate
Lane 4: HepG2 cell lysate
Lane 5: U-87 MG cell lysate
Lane 6: MCF7 cell lysate
Lane 7: Caco-2 cell lysate
Lane 8: NCI-H1299 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 63 kDa
Observed band size: 30/60 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751106) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HEK-293 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: HeLa cell lysate
Lane 4: HepG2 cell lysate
Lane 5: U-87 MG cell lysate
Lane 6: MCF7 cell lysate
Lane 7: Caco-2 cell lysate
Lane 8: NCI-H1299 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 63 kDa
Observed band size: 30/60 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751106) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.