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EIF3S1 / EIF3J Recombinant Rabbit Monoclonal Antibody [PSH06-97] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751124

EIF3S1 / EIF3J Recombinant Rabbit Monoclonal Antibody [PSH06-97] - BSA and Azide free

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

  • Rat

  • Green monkey

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

EIF3S1 / EIF3J Recombinant Rabbit Monoclonal Antibody [PSH06-97] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human EIF3J aa 1-258.

Species Reactivity

Human, Rat, Green monkey

Validated Applications

WB, IF-Cell, IHC-P, FC

Molecular Weight

Predicted band size: 29 kDa

Positive Control

HEK-293 cell lysate, HepG2 cell lysate, K-562 cell lysate, A549 cell lysate, MCF7 cell lysate, Jurkat cell lysate, HeLa cell lysate, U-87 MG cell lysate, PC-12 cell lysate, C6 cell lysate, COS-1 cell lysate, PC-12, human breast cancer tissue, rat kidney tissue.

Conjugation

unconjugated

Clone Number

PSH06-97

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IF-Cell

  • 1:100

  • IHC-P

  • 1:200-1:1,000

  • FC

  • 1:1,000

Target

Function

Eukaryotic translation initiation factor 3 subunit J (eIF3j) is a protein that in humans is encoded by the EIF3J gene. This gene encodes a core subunit of the eukaryotic initiation factor 3 complex, which participates in the initiation of translation by aiding in the recruitment of protein and mRNA components to the 40S ribosome. There are pseudogenes for this gene on chromosomes 1, 3, and 9. Alternative splicing results in multiple transcript variants encoding different isoforms.

Background References

1. Luo Y et al. Long noncoding RNA (lncRNA) EIF3J-DT induces chemoresistance of gastric cancer via autophagy activation. Autophagy. 2021 Dec

2. Egorova T et al. eIF3j facilitates loading of release factors into the ribosome. Nucleic Acids Res. 2021 Nov

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: O75822 Human

SwissProt: A0JPM9 Rat

Synonyms

eIF 3 alpha antibody

eIF-3-alpha antibody

eIF3 alpha antibody

eIF3 p35 antibody

eIF3j antibody

EIF3J_HUMAN antibody

EIF3S 1 antibody

EIF3S1 antibody

Eukaryotic translation initiation factor 3 subunit 1 antibody

Eukaryotic translation initiation factor 3 subunit J antibody

Expand

Images

  • Western blot analysis of EIF3S1 / EIF3J on different lysates with Rabbit anti-EIF3S1 / EIF3J antibody (<a href="/products/HA751124" style="font-weight: bold;text-decoration: underline;">HA751124</a>) at 1/2,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate (20 µg/Lane)<br />Lane 2: HepG2 cell lysate (20 µg/Lane)<br />Lane 3: K-562 cell lysate (20 µg/Lane)<br />Lane 4: A549 cell lysate (20 µg/Lane)<br />Lane 5: MCF7 cell lysate (20 µg/Lane)<br />Lane 6: Jurkat cell lysate (20 µg/Lane)<br />Lane 7: HeLa cell lysate (20 µg/Lane)<br />Lane 8: U-87 MG cell lysate (20 µg/Lane)<br />Lane 9: PC-12 cell lysate (20 µg/Lane)<br />Lane 10: C6 cell lysate (20 µg/Lane)<br />Lane 11: COS-1 cell lysate (20 µg/Lane)<br /><br />Predicted band size: 29 kDa<br />Observed band size: 35 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751124" style="font-weight: bold;text-decoration: underline;">HA751124</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of EIF3S1 / EIF3J on different lysates with Rabbit anti-EIF3S1 / EIF3J antibody (HA751124) at 1/2,000 dilution.

    Lane 1: HEK-293 cell lysate (20 µg/Lane)
    Lane 2: HepG2 cell lysate (20 µg/Lane)
    Lane 3: K-562 cell lysate (20 µg/Lane)
    Lane 4: A549 cell lysate (20 µg/Lane)
    Lane 5: MCF7 cell lysate (20 µg/Lane)
    Lane 6: Jurkat cell lysate (20 µg/Lane)
    Lane 7: HeLa cell lysate (20 µg/Lane)
    Lane 8: U-87 MG cell lysate (20 µg/Lane)
    Lane 9: PC-12 cell lysate (20 µg/Lane)
    Lane 10: C6 cell lysate (20 µg/Lane)
    Lane 11: COS-1 cell lysate (20 µg/Lane)

    Predicted band size: 29 kDa
    Observed band size: 35 kDa

    Exposure time: 20 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751124) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of PC-12 cells labeling EIF3S1 / EIF3J with Rabbit anti-EIF3S1 / EIF3J antibody (<a href="/products/HA751124" style="font-weight: bold;text-decoration: underline;">HA751124</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-EIF3S1 / EIF3J antibody (<a href="/products/HA751124" style="font-weight: bold;text-decoration: underline;">HA751124</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of PC-12 cells labeling EIF3S1 / EIF3J with Rabbit anti-EIF3S1 / EIF3J antibody (HA751124) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-EIF3S1 / EIF3J antibody (HA751124) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-EIF3S1 / EIF3J antibody (<a href="/products/HA751124" style="font-weight: bold;text-decoration: underline;">HA751124</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751124" style="font-weight: bold;text-decoration: underline;">HA751124</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-EIF3S1 / EIF3J antibody (HA751124) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751124) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-EIF3S1 / EIF3J antibody (<a href="/products/HA751124" style="font-weight: bold;text-decoration: underline;">HA751124</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751124" style="font-weight: bold;text-decoration: underline;">HA751124</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-EIF3S1 / EIF3J antibody (HA751124) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751124) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of PC-12 cells labeling EIF3S1 / EIF3J.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA751124" style="font-weight: bold;text-decoration: underline;">HA751124</a>, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of PC-12 cells labeling EIF3S1 / EIF3J.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA751124, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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