LATS1 / WARTS Recombinant Rabbit Monoclonal Antibody [PSH07-20] - BSA and Azide free
Catalog# HA751141
LATS1 / WARTS Recombinant Rabbit Monoclonal Antibody [PSH07-20] - BSA and Azide free
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WB
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Human
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Mouse
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Green monkey
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unconjugated
Overview
Product Name
LATS1 / WARTS Recombinant Rabbit Monoclonal Antibody [PSH07-20] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human LATS1 aa 151-600.
Species Reactivity
Human, Mouse, Green monkey
Validated Applications
WB
Target Molecular Weight
Predicted band size: 127 kDa
Positive Control
HT-29 cell lysate, K-562 cell lysate, SH-SY5Y cell lysate, HeLa cell lysate, C2C12 cell lysate, COS-1 cell lysate.
Conjugation
unconjugated
Clone Number
PSH07-20
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Large tumor suppressor kinase 1 (LATS1) is an enzyme that in humans is encoded by the LATS1 gene. It has been associated with the Hippo signaling pathway, where it phosphorylates YAP and TAZ to inactivate their function. The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced histone H1 kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.
Background References
1. Xu Y et al. The N6-methyladenosine METTL3 regulates tumorigenesis and glycolysis by mediating m6A methylation of the tumor suppressor LATS1 in breast cancer. J Exp Clin Cancer Res. 2023 Jan
2. Qi S et al. WWC proteins mediate LATS1/2 activation by Hippo kinases and imply a tumor suppression strategy. Mol Cell. 2022 May
Subcellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, spindle, microtubule organizing center, spindle pole body.
Synonyms
h-warts antibody
Large tumor suppressor homolog 1 antibody
LATS large tumor suppressor homolog 1 antibody
LATS1 antibody
LATS1_HUMAN antibody
Serine threonine protein kinase LATS1 antibody
Serine/threonine-protein kinase LATS1 antibody
WARTS antibody
WARTS protein kinase antibody
wts antibody
Images
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This data was developed using HA722808, the same antibody clone in a different buffer formulation.
Western blot analysis of LATS1 / WARTS on different lysates with Rabbit anti-LATS1 / WARTS antibody (HA722808) at 1/1,000 dilution.
Lane 1: HT-29 cell lysate
Lane 2: K-562 cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: HeLa cell lysate
Lane 5: C2C12 cell lysate
Lane 6: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 127 kDa
Observed band size: 127 kDa
Exposure time: 59 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722808) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"