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ISYNA1 Recombinant Rabbit Monoclonal Antibody [PSH07-25] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751146

ISYNA1 Recombinant Rabbit Monoclonal Antibody [PSH07-25] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

ISYNA1 Recombinant Rabbit Monoclonal Antibody [PSH07-25] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human ISYNA1 aa 1-558.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 61 kDa

Positive Control

HepG2 cell lysate, HEK-293 cell lysate, 293T cell lysate, HCT 116 cell lysate, K-562 cell lysate, Human liver tissue lysate, Mouse testis tissue lysate, Rat testis tissue lysate, human liver tissue, human testis tissue, mouse testis tissue, rat testis tissue.

Conjugation

unconjugated

Clone Number

PSH07-25

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

This gene encodes an inositol-3-phosphate synthase enzyme. The encoded protein plays a critical role in the myo-inositol biosynthesis pathway by catalyzing the rate-limiting conversion of glucose 6-phosphate to myoinositol 1-phosphate. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 4.

Background References

1. Jia Z et al. ISYNA1: An Immunomodulatory-Related Prognostic Biomarker in Colon Adenocarcinoma and Pan-Cancer. Front Cell Dev Biol. 2022 Feb

2. Yang L et al. The myo-inositol biosynthesis rate-limiting enzyme ISYNA1 suppresses the stemness of ovarian cancer via Notch1 pathway. Cell Signal. 2023 Jul

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: Q9NPH2 Human

SwissProt: Q9JHU9 Mouse

SwissProt: Q6AYK3 Rat

Synonyms

Inositol-3-phosphate synthase 1

EC:5.5.1.4

IPS 1

Myo-inositol 1-phosphate synthase

MI-1-P synthase

MIP synthase

hIPS

Myo-inositol 1-phosphate synthase A1

hINO1

ISYNA1

Expand

Images

  • This data was developed using <a href="/products/HA722813" style="font-weight: bold;text-decoration: underline;">HA722813</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of ISYNA1 on different lysates with Rabbit anti-ISYNA1 antibody (<a href="/products/HA722813" style="font-weight: bold;text-decoration: underline;">HA722813</a>) at 1/1,000 dilution.<br /><br />Lane 1: HepG2 cell lysate (20 µg/Lane)<br />Lane 2: HEK-293 cell lysate (20 µg/Lane)<br />Lane 3: 293T cell lysate (20 µg/Lane)<br />Lane 4: HCT 116 cell lysate (20 µg/Lane)<br />Lane 5: K-562 cell lysate (20 µg/Lane)<br />Lane 6: Human liver tissue lysate (40 µg/Lane)<br />Lane 7: Mouse testis tissue lysate (40 µg/Lane)<br />Lane 8: Rat testis tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 61 kDa<br />Observed band size: 55-61 kDa<br /><br />Exposure time: 6 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722813" style="font-weight: bold;text-decoration: underline;">HA722813</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    This data was developed using HA722813, the same antibody clone in a different buffer formulation.
    Western blot analysis of ISYNA1 on different lysates with Rabbit anti-ISYNA1 antibody (HA722813) at 1/1,000 dilution.

    Lane 1: HepG2 cell lysate (20 µg/Lane)
    Lane 2: HEK-293 cell lysate (20 µg/Lane)
    Lane 3: 293T cell lysate (20 µg/Lane)
    Lane 4: HCT 116 cell lysate (20 µg/Lane)
    Lane 5: K-562 cell lysate (20 µg/Lane)
    Lane 6: Human liver tissue lysate (40 µg/Lane)
    Lane 7: Mouse testis tissue lysate (40 µg/Lane)
    Lane 8: Rat testis tissue lysate (40 µg/Lane)

    Predicted band size: 61 kDa
    Observed band size: 55-61 kDa

    Exposure time: 6 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722813) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • This data was developed using <a href="/products/HA722813" style="font-weight: bold;text-decoration: underline;">HA722813</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-ISYNA1 antibody (<a href="/products/HA722813" style="font-weight: bold;text-decoration: underline;">HA722813</a>) at 1/10,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722813" style="font-weight: bold;text-decoration: underline;">HA722813</a>) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA722813, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-ISYNA1 antibody (HA722813) at 1/10,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722813) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA722813" style="font-weight: bold;text-decoration: underline;">HA722813</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-ISYNA1 antibody (<a href="/products/HA722813" style="font-weight: bold;text-decoration: underline;">HA722813</a>) at 1/10,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722813" style="font-weight: bold;text-decoration: underline;">HA722813</a>) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA722813, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-ISYNA1 antibody (HA722813) at 1/10,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722813) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA722813" style="font-weight: bold;text-decoration: underline;">HA722813</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-ISYNA1 antibody (<a href="/products/HA722813" style="font-weight: bold;text-decoration: underline;">HA722813</a>) at 1/10,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722813" style="font-weight: bold;text-decoration: underline;">HA722813</a>) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA722813, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-ISYNA1 antibody (HA722813) at 1/10,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722813) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA722813" style="font-weight: bold;text-decoration: underline;">HA722813</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-ISYNA1 antibody (<a href="/products/HA722813" style="font-weight: bold;text-decoration: underline;">HA722813</a>) at 1/10,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722813" style="font-weight: bold;text-decoration: underline;">HA722813</a>) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA722813, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-ISYNA1 antibody (HA722813) at 1/10,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722813) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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