CAMKK2 Recombinant Rabbit Monoclonal Antibody [PSH07-28] - BSA and Azide free
Catalog# HA751149
CAMKK2 Recombinant Rabbit Monoclonal Antibody [PSH07-28] - BSA and Azide free
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WB
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Human
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unconjugated
Overview
Product Name
CAMKK2 Recombinant Rabbit Monoclonal Antibody [PSH07-28] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 65 kDa
Positive Control
LNCaP cell lysate, HL-60 cell lysate, SH-SY5Y cell lysate, THP-1 cell lysate, 293T cell lysate.
Conjugation
unconjugated
Clone Number
PSH07-28
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Calcium/calmodulin-dependent protein kinase kinase 2 is an enzyme that in humans is encoded by the CAMKK2 gene. The product of this gene belongs to the serine/threonine-specific protein kinase family, and to the Ca++/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4, which increases their catalytic activity. CaMK1 and CaMK4 are phosphorylated at the Thr 177 and Thr 196 resides respectively.
Background References
1. Kaiser J et al. CaMKK2 as an emerging treatment target for bipolar disorder. Mol Psychiatry. 2023 Nov
2. Negoita F et al. CaMKK2 is not involved in contraction-stimulated AMPK activation and glucose uptake in skeletal muscle. Mol Metab. 2023 Sep
Subcellular Location
Nucleus, Cytoplasm, Cell projection, neuron projection.
UNIPROT
Synonyms
Calcium/calmodulin dependent protein kinase beta antibody
Calcium/calmodulin dependent protein kinase kinase 2 antibody
Calcium/calmodulin dependent protein kinase kinase 2 beta antibody
Calcium/calmodulin dependent protein kinase kinase beta antibody
Calcium/calmodulin-dependent protein kinase kinase 2 antibody
Calcium/calmodulin-dependent protein kinase kinase beta antibody
CaM kinase kinase beta antibody
CaM KK beta antibody
CaM-kinase kinase 2 antibody
CaM-kinase kinase beta antibody
ExpandImages
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This data was developed using HA722816, the same antibody clone in a different buffer formulation.
Western blot analysis of CAMKK2 on different lysates with Rabbit anti-CAMKK2 antibody (HA722816) at 1/1,000 dilution.
Lane 1: LNCaP cell lysate
Lane 2: HL-60 cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: THP-1 cell lysate
Lane 5: 293T cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 65 kDa
Observed band size: 65 kDa
Exposure time: 59 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722816) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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