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ERO1L Recombinant Rabbit Monoclonal Antibody [PSH07-30] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751151

ERO1L Recombinant Rabbit Monoclonal Antibody [PSH07-30] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

ERO1L Recombinant Rabbit Monoclonal Antibody [PSH07-30] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human ERO1L aa 1-468.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 54 kDa

Positive Control

HeLa cell lysate, HepG2 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, L6 cell lysate, Mouse placenta tissue lysate, human colon tissue.

Conjugation

unconjugated

Clone Number

PSH07-30

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

Target

Function

ERO1-like protein alpha is a protein that in humans is encoded by the ERO1L gene. Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell. Required for the proper folding of immunoglobulins 1. Plays an important role in ER stress-induced, CHOP-dependent apoptosis by activating the inositol 1,4,5-trisphosphate receptor IP3R1. Involved in the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin.

Background References

1. Liu L et al. ERO1L Is a Novel and Potential Biomarker in Lung Adenocarcinoma and Shapes the Immune-Suppressive Tumor Microenvironment. Front Immunol. 2021 Jul

2. Xie J et al. ERO1L promotes the proliferation and metastasis of lung adenocarcinoma via the Wnt2/beta-catenin signaling pathway. Mol Carcinog. 2022 Oct

Subcellular Location

Endoplasmic reticulum membrane, membrane protein, Golgi apparatus lumen, Secreted, Cell projection, dendrite.

UNIPROT

SwissProt: Q96HE7 Human

SwissProt: Q8R180 Mouse

SwissProt: Q8R4A1 Rat

Synonyms

Endoplasmic oxidoreductin 1 like protein antibody

Endoplasmic oxidoreductin-1-like protein antibody

Endoplasmic reticulum oxidoreductase 1 alpha antibody

Endoplasmic reticulum oxidoreductin 1-like antibody

ERO1 alpha antibody

ERO1 L antibody

ERO1 Lalpha antibody

ERO1 like protein alpha antibody

ERO1-alpha antibody

ERO1-L antibody

Expand

Images

  • Western blot analysis of ERO1L on different lysates with Rabbit anti-ERO1L antibody (<a href="/products/HA751151" style="font-weight: bold;text-decoration: underline;">HA751151</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: HepG2 cell lysate (20 µg/Lane)<br />Lane 3: Jurkat cell lysate (20 µg/Lane)<br />Lane 4: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 5: L6 cell lysate (20 µg/Lane)<br />Lane 6: Mouse placenta tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 54 kDa<br />Observed band size: 60 kDa<br /><br />Exposure time: 2 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751151" style="font-weight: bold;text-decoration: underline;">HA751151</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ERO1L on different lysates with Rabbit anti-ERO1L antibody (HA751151) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: HepG2 cell lysate (20 µg/Lane)
    Lane 3: Jurkat cell lysate (20 µg/Lane)
    Lane 4: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 5: L6 cell lysate (20 µg/Lane)
    Lane 6: Mouse placenta tissue lysate (40 µg/Lane)

    Predicted band size: 54 kDa
    Observed band size: 60 kDa

    Exposure time: 2 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751151) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-ERO1L antibody (<a href="/products/HA751151" style="font-weight: bold;text-decoration: underline;">HA751151</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751151" style="font-weight: bold;text-decoration: underline;">HA751151</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-ERO1L antibody (HA751151) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751151) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"