IRG1 Recombinant Rabbit Monoclonal Antibody [PSH07-31] - BSA and Azide free
Catalog# HA751152
IRG1 Recombinant Rabbit Monoclonal Antibody [PSH07-31] - BSA and Azide free
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WB
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IF-Cell
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Mouse
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unconjugated
Overview
Product Name
IRG1 Recombinant Rabbit Monoclonal Antibody [PSH07-31] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within mouse IRG1 aa 1-488.
Species Reactivity
Mouse
Validated Applications
WB, IF-Cell
Target Molecular Weight
Predicted band size: 54 kDa
Positive Control
RAW264.7 treated with 0.1μg/mL LPS for 6 hours cell lysate, J774A.1 treated with 1μg/mL LPS for 24 hours cell lysate, mBMMC treated with 0.1μg/mL LPS and 10ng/mL IFN-gamma for 24 hours cell lysate, mBMSC treated with 40ng/mL MCSF for 7 days then add 100ng/mL LPS for 48 hours cell lysate, RAW264.7 cells treated with 0.1μg/mL LPS for 6 hours.
Conjugation
unconjugated
Clone Number
PSH07-31
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:100
Target
Function
Cis-aconitate decarboxylase that catalyzes production of itaconate and is involved in the inhibition of the inflammatory response. Acts as a negative regulator of the Toll-like receptors (TLRs)-mediated inflammatory innate response by stimulating the tumor necrosis factor alpha-induced protein TNFAIP3 expression via reactive oxygen species (ROS) in LPS-tolerized macrophages. Involved in antimicrobial response of innate immune cells; ACOD1-mediated itaconic acid production contributes to the antimicrobial activity of macrophages by generating itaconate, leading to alkylation of proteins, such as TFEB. Involved in antiviral response following infection by flavivirus in neurons: ACOD1-mediated itaconate production inhibits the activity of succinate dehydrogenase, generating a metabolic state in neurons that suppresses replication of viral genomes (By similarity). Plays a role in the embryo implantation (By similarity).
Background References
1. Wu R et al. The Dual Role of ACOD1 in Inflammation. J Immunol. 2023 Aug
2. Wu R et al. Mitochondrial ACOD1/IRG1 in infection and sterile inflammation. J Intensive Med. 2022 Feb
Subcellular Location
Mitochondrion.
UNIPROT
Synonyms
ACOD1 antibody
Aconitate decarboxylase 1 antibody
Aconitate decarboxylase antibody
CAD antibody
Cis aconitate decarboxylase antibody
Immune responsive gene 1 protein homolog antibody
Immune-responsive gene 1 protein homolog antibody
Immunoresponsive 1 homolog (mouse) antibody
Irg1 antibody
IRG1_HUMAN antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of IRG1 on different lysates with Rabbit anti-IRG1 antibody (HA751152) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.
Lane 1: RAW264.7 cell lysate
Lane 2: RAW264.7 treated with 0.1μg/mL LPS for 6 hours cell lysate
Lane 3: J774A.1 cell lysate
Lane 4: J774A.1 treated with 1μg/mL LPS for 24 hours cell lysate
Lane 5: mBMMC cell lysate
Lane 6: mBMMC treated with 0.1μg/mL LPS and 10ng/mL IFN-gamma for 24 hours cell lysate
Lane 7: mBMSC cell lysate
Lane 8: mBMSC treated with 40ng/mL MCSF for 7 days then add 100ng/mL LPS for 48 hours cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751152) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Immunocytochemistry analysis of RAW264.7 cells treated with 0.1μg/mL LPS for 6 hours labeling IRG1 with Rabbit anti-IRG1 antibody (HA751152) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IRG1 antibody (HA751152) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"