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Filamin B Recombinant Rabbit Monoclonal Antibody [PSH07-34] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751155

Filamin B Recombinant Rabbit Monoclonal Antibody [PSH07-34] - BSA and Azide free

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Filamin B Recombinant Rabbit Monoclonal Antibody [PSH07-34] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Filamin B aa

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 278 kDa

Positive Control

HeLa cell lysate, A549 cell lysate, A431 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Rat lung tissue lysate, human colon tissue, rat colon tissue.

Conjugation

unconjugated

Clone Number

PSH07-34

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:5,000

  • IF-Tissue

  • 1:1,000

Target

Function

Filamin B, beta (FLNB), also known as Filamin B, beta (truncated actin binding protein 278 homolog), is a cytoplasmic protein which in humans is encoded by the FLNB gene. FLNB regulates intracellular communication and signalling by cross-linking the protein actin to allow direct communication between the cell membrane and cytoskeletal network, to control and guide proper skeletal development. Mutations in the FLNB gene are involved in several lethal bone dysplasias, including boomerang dysplasia and atelosteogenesis type I.

Background References

1. Zhao X et al. Actin-binding protein filamin B regulates the cell-surface retention of endothelial sphingosine 1-phosphate receptor 1. J Biol Chem. 2023 Jul

2. Wu H et al. Cell-Dependent Pathogenic Roles of Filamin B in Different Skeletal Malformations. Oxid Med Cell Longev. 2022 Jul

Subcellular Location

Cytoplasm, cell cortex, cytoskeleton, stress, myofibril, sarcomere, Z line.

UNIPROT

SwissProt: O75369 Human

SwissProt: Q80X90 Mouse

Entrez Gene: 306204 Rat

Synonyms

ABP 278 antibody

ABP 280 homolog antibody

ABP-278 antibody

ABP-280 homolog antibody

ABP278 antibody

Actin binding like protein antibody

Actin binding protein 278 antibody

Actin-binding-like protein antibody

AOI antibody

Beta filamin antibody

Expand

Images

  • Western blot analysis of Filamin B on different lysates with Rabbit anti-Filamin B antibody (<a href="/products/HA751155" style="font-weight: bold;text-decoration: underline;">HA751155</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: A549 cell lysate (20 µg/Lane)<br />Lane 3: A431 cell lysate (20 µg/Lane)<br />Lane 4: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 5: PC-12 cell lysate (20 µg/Lane)<br />Lane 6: Rat lung tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 278 kDa<br />Observed band size: 315 kDa<br /><br />Exposure time: Lane 1-3: 4 seconds; Lane 4-6: 42 seconds; ECL: K1801;<br /><br />3-8% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751155" style="font-weight: bold;text-decoration: underline;">HA751155</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Filamin B on different lysates with Rabbit anti-Filamin B antibody (HA751155) at 1/5,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: A549 cell lysate (20 µg/Lane)
    Lane 3: A431 cell lysate (20 µg/Lane)
    Lane 4: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 5: PC-12 cell lysate (20 µg/Lane)
    Lane 6: Rat lung tissue lysate (40 µg/Lane)

    Predicted band size: 278 kDa
    Observed band size: 315 kDa

    Exposure time: Lane 1-3: 4 seconds; Lane 4-6: 42 seconds; ECL: K1801;

    3-8% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751155) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Filamin B antibody (<a href="/products/HA751155" style="font-weight: bold;text-decoration: underline;">HA751155</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751155" style="font-weight: bold;text-decoration: underline;">HA751155</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Filamin B antibody (HA751155) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751155) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-Filamin B antibody (<a href="/products/HA751155" style="font-weight: bold;text-decoration: underline;">HA751155</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751155" style="font-weight: bold;text-decoration: underline;">HA751155</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-Filamin B antibody (HA751155) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751155) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Products with the same target and pathway

metafield image

Filamin B Recombinant Rabbit Monoclonal Antibody [PSH07-34]

Application: WB,IHC-P,IF-Tissue

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated