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SLUG Recombinant Rabbit Monoclonal Antibody [PSH07-40] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751161

SLUG Recombinant Rabbit Monoclonal Antibody [PSH07-40] - BSA and Azide free

Application

  • WB

  • IF-Cell

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

SLUG Recombinant Rabbit Monoclonal Antibody [PSH07-40] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human SLUG aa 1-150.

Species Reactivity

Human

Validated Applications

WB, IF-Cell

Molecular Weight

Predicted band size: 30 kDa

Positive Control

U-87 MG cell lysate, SK-MEL-28 cell lysate, SK-MEL-28.

Conjugation

unconjugated

Clone Number

PSH07-40

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:100

Target

Function

This gene encodes a member of the Snail superfamily of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. It regulates differentiation and migration of neural crest cells along with other genes (e.g. FOXD3, SOX9 and SOX10, BMPs) in embryonic life. Mutations in this gene may be associated with sporadic cases of neural tube defects. SNAI2 downregulates expression of E-cadherin in premigratory neural crest cells; thus, SNAI2 induces tightly bound epithelial cells to break into a loose mesenchymal phenotype, allowing gastrulation of mesoderm in the developing embryo. Structurally similar to anti-apoptotic Ces-1 in C. elegans, SLUG is a negative regulator of productive cell death in the developing embryo and adults.

Background References

1. Li W et al. Deubiquitinase USP20 promotes breast cancer metastasis by stabilizing SNAI2. Genes Dev. 2020 Oct

2. Jin Y et al. SNAI2 promotes the development of ovarian cancer through regulating ferroptosis. Bioengineered. 2022 Mar

Subcellular Location

Nucleus, Cytoplasm.

UNIPROT

SwissProt: O43623 Human

Synonyms

MGC10182 antibody

Neural crest transcription factor Slug antibody

Protein snail homolog 2 antibody

Slug (chicken homolog) zinc finger protein antibody

Slug homolog zinc finger protein antibody

Slug zinc finger protein antibody

SLUGH antibody

SLUGH 1 antibody

SLUGH1 antibody

SNAI 2 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of SLUG on different lysates with Rabbit anti-SLUG antibody (<a href="/products/HA751161" style="font-weight: bold;text-decoration: underline;">HA751161</a>) at 1/1,000 dilution.<br /><br />Lane 1: U-87 MG cell lysate<br />Lane 2: MCF7 cell lysate (negative)<br />Lane 3: SK-MEL-28 cell lysate<br />Lane 4: PANC-1 cell lysate (low expression)<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 30 kDa<br />Observed band size: 35 kDa<br /><br />Exposure time: 2 minutes 7 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751161" style="font-weight: bold;text-decoration: underline;">HA751161</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of SLUG on different lysates with Rabbit anti-SLUG antibody (HA751161) at 1/1,000 dilution.

    Lane 1: U-87 MG cell lysate
    Lane 2: MCF7 cell lysate (negative)
    Lane 3: SK-MEL-28 cell lysate
    Lane 4: PANC-1 cell lysate (low expression)

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 30 kDa
    Observed band size: 35 kDa

    Exposure time: 2 minutes 7 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751161) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunocytochemistry analysis of SK-MEL-28 (positive) and PANC-1 (low expression) labeling SLUG with Rabbit anti-SLUG antibody (<a href="/products/HA751161" style="font-weight: bold;text-decoration: underline;">HA751161</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SLUG antibody (<a href="/products/HA751161" style="font-weight: bold;text-decoration: underline;">HA751161</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    ☑ Relative expression (RE)

    Immunocytochemistry analysis of SK-MEL-28 (positive) and PANC-1 (low expression) labeling SLUG with Rabbit anti-SLUG antibody (HA751161) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SLUG antibody (HA751161) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Products with the same target and pathway

metafield image

SLUG Recombinant Rabbit Monoclonal Antibody [PSH07-40]

Application: WB,IF-Cell

Reactivity: Human

Conjugate: unconjugated