Mono-Methyl Arginine (R*GG) Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH07-52] (HA751173)
Catalog# HA751173
Rabbit Monoclonal to Mono-Methyl Arginine (R*GG)
Tested for
-
WB
Reacts with
-
Species independent
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Mono-Methyl Arginine (R*GG) on different lysates with Rabbit anti-Mono-Methyl Arginine (R*GG) antibody (HA751173) at 1/1,000 dilution.
Lane 1: 293T cell lysate
Lane 2: 293T treated with 10nM Type I PRMT inhibitor MS023 for 48 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Observed band size: Mutiple kDa
Exposure time: 5 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751173) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Mono-Methyl Arginine (R*GG) on different lysates with Rabbit anti-Mono-Methyl Arginine (R*GG) antibody (HA751173) at 1/1,000 dilution.
Lane 1: 293T cell lysate
Lane 2: 293T treated with 10nM Type I PRMT inhibitor MS023 for 48 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Observed band size: Mutiple kDa
Exposure time: 5 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751173) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.