BRCA2 Recombinant Rabbit Monoclonal Antibody [PSH07-55] - BSA and Azide free
Catalog# HA751177
BRCA2 Recombinant Rabbit Monoclonal Antibody [PSH07-55] - BSA and Azide free
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WB
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FC
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Human
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unconjugated
Overview
Product Name
BRCA2 Recombinant Rabbit Monoclonal Antibody [PSH07-55] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human BRCA2 aa 3,219-3,418.
Species Reactivity
Human
Validated Applications
WB, FC
Target Molecular Weight
Predicted band size: 384 kDa
Positive Control
HeLa cell lysate, 293T cell lysate, Jurkat cell lysate, MCF7.
Conjugation
unconjugated
Clone Number
PSH07-55
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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FC
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1:1,000
Target
Function
Although the structures of the BRCA1 and BRCA2 genes are very different, at least some functions are interrelated. The proteins made by both genes are essential for repairing damaged DNA. BRCA2 binds the single strand DNA and directly interacts with the recombinase RAD51 to stimulate and maintain strand invasion, a vital step of homologous recombination. The localization of RAD51 to the DNA double-strand break requires the formation of the BRCA1-PALB2-BRCA2 complex. PALB2 (Partner and localizer of BRCA2) can function synergistically with a BRCA2 chimera (termed piccolo, or piBRCA2) to further promote strand invasion. These breaks can be caused by natural and medical radiation or other environmental exposures, but also occur when chromosomes exchange genetic material during a special type of cell division that creates sperm and eggs (meiosis). Double strand breaks are also generated during repair of DNA cross links. By repairing DNA, these proteins play a role in maintaining the stability of the human genome and prevent dangerous gene rearrangements that can lead to hematologic and other cancers.
Background References
1. Xie C et al. BRCA2 gene mutation in cancer. Medicine (Baltimore). 2022 Nov
2. Samstein RM et al. Mutations in BRCA1 and BRCA2 differentially affect the tumor microenvironment and response to checkpoint blockade immunotherapy. Nat Cancer. 2021 Dec
Subcellular Location
Nucleus, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
UNIPROT
Synonyms
BRCA 2 antibody
BRCA1/BRCA2 containing complex subunit 2 antibody
Brca2 antibody
BRCA2, DNA repair associated antibody
BRCA2_HUMAN antibody
BRCC 2 antibody
BRCC2 antibody
Breast and ovarian cancer susceptibility gene early onset antibody
breast and ovarian cancer susceptibility protein 2 antibody
Breast cancer 2 early onset antibody
ExpandImages
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Western blot analysis of BRCA2 on different lysates with Rabbit anti-BRCA2 antibody (HA751177) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: Jurkat cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 384 kDa
Observed band size: 460 kDa
Exposure time: 25 seconds; ECL: K1802;
3-8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751177) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Flow cytometric analysis of MCF7 cells labeling BRCA2.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA751177, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"