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LYVE1 Recombinant Rabbit Monoclonal Antibody [PSH07-67] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751179

LYVE1 Recombinant Rabbit Monoclonal Antibody [PSH07-67] - BSA and Azide free

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Mouse

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

LYVE1 Recombinant Rabbit Monoclonal Antibody [PSH07-67] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse LYVE1 aa 1-250.

Species Reactivity

Mouse

Validated Applications

WB, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 35 kDa

Positive Control

Mouse lung tissue lysate, mouse colon tissue.

Conjugation

unconjugated

Clone Number

PSH07-67

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

  • IF-Tissue

  • 1:100

Target

Function

Lymphatic vessel endothelial hyaluronan receptor 1 (LYVE1), also known as extracellular link domain containing 1 (XLKD1) is a Link domain-containing hyaladherin, a protein capable of binding to hyaluronic acid (HA), homologous to CD44, the main HA receptor. In humans it is encoded by the LYVE1 gene. LYVE1 is a type I integral membrane glycoprotein. It acts as a receptor and binds to both soluble and immobilized hyaluronan. This protein may function in lymphatic hyaluronan transport and have a role in tumor metastasis. LYVE-1 is a cell surface receptor on lymphatic endothelial cells that can be used as a lymphatic endothelial cell marker, allowing for the isolation of these cells for experimental purposes. The physiological role for this receptor is still the subject of debate, but evolutionary conservation suggests an important role. Expression of LYVE1 not restricted to lymph vessels but is also observed in normal liver blood sinusoids, and embryonic blood vessels.

Background References

1. Yu X et al. Septal LYVE1(+) macrophages control adipocyte stem cell adipogenic potential. Science. 2025 Aug

2. Siret C et al. Deciphering the heterogeneity of the Lyve1(+) perivascular macrophages in the mouse brain. Nat Commun. 2022 Nov

Subcellular Location

Membrane.

UNIPROT

SwissProt: Q8BHC0 Mouse

Synonyms

Cell surface retention sequence-binding protein 1 antibody

CRSBP 1 antibody

CRSBP-1 antibody

CRSBP1 antibody

extracellular link domain containing 1 antibody

extracellular link domain-containing 1 antibody

Extracellular link domain-containing protein 1 antibody

HAR antibody

Hyaluronic acid receptor antibody

Lymphatic endothelium specific hyaluronan receptor antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of LYVE1 on different lysates with Rabbit anti-LYVE1 antibody (<a href="/products/HA751179" style="font-weight: bold;text-decoration: underline;">HA751179</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse lung tissue lysate<br />Lane 2: Mouse brain tissue lysate (negative)<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 35 kDa<br />Observed band size: 60 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751179" style="font-weight: bold;text-decoration: underline;">HA751179</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of LYVE1 on different lysates with Rabbit anti-LYVE1 antibody (HA751179) at 1/1,000 dilution.

    Lane 1: Mouse lung tissue lysate
    Lane 2: Mouse brain tissue lysate (negative)

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 35 kDa
    Observed band size: 60 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751179) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-LYVE1 antibody (<a href="/products/HA751179" style="font-weight: bold;text-decoration: underline;">HA751179</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751179" style="font-weight: bold;text-decoration: underline;">HA751179</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-LYVE1 antibody (HA751179) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751179) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: Immunofluorescence (IF-tissue)<br /><br />Species: Mouse<br />Tissue: Colon<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× PBS<br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA751179" style="font-weight: bold;text-decoration: underline;">HA751179</a>, 1/100, overnight at 4℃.<br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IF-tissue)

    Species: Mouse
    Tissue: Colon
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× PBS
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA751179, 1/100, overnight at 4℃.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"