Pet1 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH07-77] (HA751189)
Catalog# HA751189
Rabbit Monoclonal to Pet1
Tested for
-
WB
-
IF-Cell
-
IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Pet1 on different lysates with Rabbit anti-Pet1 antibody (HA751189) at 1/2,000 dilution.
Lane 1: 293T transfected with empty control cell lysate
Lane 2: 293T transfected with Pet1 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 25 kDa
Observed band size: 30 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751189) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Pet1 on different lysates with Rabbit anti-Pet1 antibody (HA751189) at 1/2,000 dilution.
Lane 1: 293T transfected with empty control cell lysate
Lane 2: 293T transfected with Pet1 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 25 kDa
Observed band size: 30 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751189) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.