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LRRK2 Recombinant Rabbit Monoclonal Antibody [PSH07-96] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751198

LRRK2 Recombinant Rabbit Monoclonal Antibody [PSH07-96] - BSA and Azide free

Application

  • IHC-P

  • IF-Tissue

  • IHC-Fr

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Predicted reactivity

  • Cynomolgus monkey

  • Pig

Conjugation

  • unconjugated

Overview

Product Name

LRRK2 Recombinant Rabbit Monoclonal Antibody [PSH07-96] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human LRRK2 aa 1,301-1,600.

Species Reactivity

Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

IHC-P, IF-Tissue, IHC-Fr

Target Molecular Weight

Predicted band size: 286 kDa

Positive Control

Human brain tissue, mouse brain tissue, rat brain tissue.

Conjugation

unconjugated

Clone Number

PSH07-96

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

The LRRK2 gene encodes a protein with an armadillo repeats (ARM) region, an ankyrin repeat (ANK) region, a leucine-rich repeat (LRR) domain, a kinase domain, a RAS domain, a GTPase domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. LRRK2 interacts with the C-terminal R2 RING finger domain of parkin, and parkin interacted with the COR domain of LRRK2. Expression of mutant LRRK2 induced apoptotic cell death in neuroblastoma cells and in mouse cortical neurons. Expression of LRRK2 mutants implicated in autosomal dominant Parkinson's disease causes shortening and simplification of the dendritic tree in vivo and in cultured neurons. This is mediated in part by alterations in macroautophagy, and can be prevented by protein kinase A regulation of the autophagy protein LC3. The G2019S and R1441C mutations elicit post-synaptic calcium imbalance, leading to excess mitochondrial clearance from dendrites by mitophagy. LRRK2 is also a substrate for chaperone-mediated autophagy.

Background References

1. Usmani A et al. The Cell Biology of LRRK2 in Parkinson\'s Disease. Mol Cell Biol. 2021 Apr

2. Rocha EM et al. LRRK2 and idiopathic Parkinson\'s disease. Trends Neurosci. 2022 Mar

Subcellular Location

Cytoplasmic vesicle, Perikaryon, Golgi apparatus membrane, Cell projection, axon, dendrite, Endoplasmic reticulum membrane, secretory vesicle, synaptic vesicle membrane, Endosome, Lysosome, Mitochondrion outer membrane, Cytoplasm, cytoskeleton.

UNIPROT

SwissProt: Q5S007 Human

SwissProt: Q5S006 Mouse

Entrez Gene: 300160 Rat

Synonyms

augmented in rheumatoid arthritis 17 antibody

AURA17 antibody

Dardarin antibody

Leucine rich repeat kinase 2 antibody

leucine rich repeat serine threonine protein kinase 2 antibody

Leucine-rich repeat serine/threonine-protein kinase 2 antibody

LRRK 2 antibody

LRRK2 antibody

LRRK2_HUMAN antibody

PARK 8 antibody

Expand

Images

  • This data was developed using <a href="/products/HA722932" style="font-weight: bold;text-decoration: underline;">HA722932</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-LRRK2 antibody (<a href="/products/HA722932" style="font-weight: bold;text-decoration: underline;">HA722932</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722932" style="font-weight: bold;text-decoration: underline;">HA722932</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA722932, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-LRRK2 antibody (HA722932) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722932) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA722932" style="font-weight: bold;text-decoration: underline;">HA722932</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-LRRK2 antibody (<a href="/products/HA722932" style="font-weight: bold;text-decoration: underline;">HA722932</a>) at 1/10,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722932" style="font-weight: bold;text-decoration: underline;">HA722932</a>) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA722932, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-LRRK2 antibody (HA722932) at 1/10,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722932) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA722932" style="font-weight: bold;text-decoration: underline;">HA722932</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-LRRK2 antibody (<a href="/products/HA722932" style="font-weight: bold;text-decoration: underline;">HA722932</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722932" style="font-weight: bold;text-decoration: underline;">HA722932</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA722932, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-LRRK2 antibody (HA722932) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722932) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA722932" style="font-weight: bold;text-decoration: underline;">HA722932</a>, the same antibody clone in a different buffer formulation.<br />Application: IF-tissue<br /><br />Species: Mouse<br /><br />Site: Cerebral cortex<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/200

    This data was developed using HA722932, the same antibody clone in a different buffer formulation.
    Application: IF-tissue

    Species: Mouse

    Site: Cerebral cortex

    Sample: Paraffin-embedded section

    Antibody concentration: 1/200

  • This data was developed using <a href="/products/HA722932" style="font-weight: bold;text-decoration: underline;">HA722932</a>, the same antibody clone in a different buffer formulation.<br />Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebral cortex<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/200<br /><br />Antigen retrieval: Not required

    This data was developed using HA722932, the same antibody clone in a different buffer formulation.
    Application: IHC-Fr

    Species: Mouse

    Site: Cerebral cortex

    Sample: Frozen section

    Antibody concentration: 1/200

    Antigen retrieval: Not required

  • This data was developed using <a href="/products/HA722932" style="font-weight: bold;text-decoration: underline;">HA722932</a>, the same antibody clone in a different buffer formulation.<br />Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: Cerebral cortex<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/200<br /><br />Antigen retrieval: Not required

    This data was developed using HA722932, the same antibody clone in a different buffer formulation.
    Application: IHC-Fr

    Species: Rat

    Site: Cerebral cortex

    Sample: Frozen section

    Antibody concentration: 1/200

    Antigen retrieval: Not required

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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LRRK2 Recombinant Rabbit Monoclonal Antibody [PSH07-96]

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Conjugate: unconjugated