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CD41 Recombinant Rabbit Monoclonal Antibody [PSH08-02] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751202

CD41 Recombinant Rabbit Monoclonal Antibody [PSH08-02] - BSA and Azide free

Application

  • IHC-P

  • FC

  • IF-Cell

  • Depletion

Reactivity

  • Mouse

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

CD41 Recombinant Rabbit Monoclonal Antibody [PSH08-02] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse CD41 aa 451-989.

Species Reactivity

Mouse

Validated Applications

IHC-P, FC, IF-Cell, Depletion

Molecular Weight

Predicted band size: 113 kDa

Positive Control

Mouse placenta tissue, mouse spleen tissue, mouse platelets.

Conjugation

unconjugated

Clone Number

PSH08-02

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:200-1:1,000

  • FC

  • 1:1,000

  • IF-Cell

  • 1:1,000

Target

Function

Integrin alpha-IIb is a protein that in humans is encoded by the ITGA2B gene. ITGA2B, also known as CD41, encodes integrin alpha chain 2b. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 2b undergoes post-translational cleavage to yield disulfide-linked light and heavy chains that join with beta 3 to form a fibrinogen receptor expressed in platelets that plays a crucial role in coagulation. Mutations that interfere with this role result in thrombasthenia. At least 38 disease-causing mutations in this gene have been discovered. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.

Background References

1. Dai L et al. Characterization of CD41(+) cells in the lymph node. Front Immunol. 2022 Aug

2. Sugimoto K et al. CD41(+) extracellular vesicles produced by avian thrombocytes contain microRNAs. Genes Cells. 2023 Dec

Subcellular Location

Membrane.

UNIPROT

SwissProt: Q9QUM0 Mouse

Synonyms

antigen CD41 antibody

BDPLT16 antibody

BDPLT2 antibody

CD41 antibody

CD41B antibody

form 2 antibody

GP2B antibody

GPalpha IIb antibody

GPIIb antibody

GT antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded mouse placenta tissue with Rabbit anti-CD41 antibody (<a href="/products/HA751202" style="font-weight: bold;text-decoration: underline;">HA751202</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751202" style="font-weight: bold;text-decoration: underline;">HA751202</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue with Rabbit anti-CD41 antibody (HA751202) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751202) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CD41 antibody (<a href="/products/HA751202" style="font-weight: bold;text-decoration: underline;">HA751202</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751202" style="font-weight: bold;text-decoration: underline;">HA751202</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CD41 antibody (HA751202) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751202) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of mouse platelets labeling CD41.<br /><br />Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (<a href="/products/HA751202" style="font-weight: bold;text-decoration: underline;">HA751202</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of mouse platelets labeling CD41.

    Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA751202, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Immunocytochemistry analysis of mouse platelets labeling CD41 with Rabbit anti-CD41 antibody (<a href="/products/HA751202" style="font-weight: bold;text-decoration: underline;">HA751202</a>, cyan) at 1/1,000 dilution.

    Immunocytochemistry analysis of mouse platelets labeling CD41 with Rabbit anti-CD41 antibody (HA751202, cyan) at 1/1,000 dilution.

  • Validation of anti-CD41 antibody depletion function in vivo.

    Validation of anti-CD41 antibody depletion function in vivo.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"